Yue Tao, Zhang Ping, Liu Peng, Deng Qing-Li, Ji Qiong-Mei, Li Xiu-Ying, Zhu Zhen-Yu
Department of Biochemistry, Sun Yat-sen Medical College, Sun Yat-sen University, Guangzhou, Guangdong, 510080, PR China.
Ai Zheng. 2003 Feb;22(2):148-51.
BACKGROUND & OBJECTIVE: Tumor angiogenesis play an important role in growth and metastasis of cancer. Angiogenesis inhibitors induce apoptosis in cancer by inhibiting tumor angiogenesis and have strong inhibiting effect on both growth and metastasis of cancer. This study was designed to explore the effect of transfection of human endostatin gene on nasopharyngeal carcinoma CNE2 cells xenograft growth in nude mice.
The plasmids (pBlast-hIL-hEndostatin, pBlast-hEndostatin, and pBlast-MCS) were transfected and lipofectin-mediated into the CNE2 cell line. The biological activity of secreted hEndostain from gene-transferred cell lines was determined using MTT method in vitro. Then the transfected CNE2 cells were injected into the nude mice and tumorigenicity of CNE2 was observed in vivo.
The supernatant of CNE2 cell transfected with pBlast-hIL-hEndostatin effectively inhibited the growth of endothelial cell (ECV304). The volume and the weight of tumor in pBlast-hIL -hEndostatin transfecting cells group were less than those in control group (P< 0.01). The growth speed of tumor in pBlast-hIL-hEndostatin transfecting cells group was slower than that in control group.
Transfection of hEndostatin gene could inhibit CNE2 cell growth in nude mice.
肿瘤血管生成在癌症的生长和转移中起重要作用。血管生成抑制剂通过抑制肿瘤血管生成诱导癌细胞凋亡，对癌症的生长和转移均有较强的抑制作用。本研究旨在探讨人内皮抑素基因转染对鼻咽癌CNE2细胞裸鼠移植瘤生长的影响。
将质粒（pBlast-hIL-hEndostatin、pBlast-hEndostatin和pBlast-MCS）用脂质体介导转染入CNE2细胞系。采用MTT法体外测定基因转染细胞系分泌的hEndostain的生物学活性。然后将转染后的CNE2细胞注射到裸鼠体内，观察CNE2的致瘤性。
pBlast-hIL-hEndostatin转染的CNE2细胞培养上清能有效抑制内皮细胞（ECV304）的生长。pBlast-hIL -hEndostatin转染细胞组肿瘤的体积和重量均小于对照组（P<0.01）。pBlast-hIL-hEndostatin转染细胞组肿瘤的生长速度比对照组慢。
内皮抑素基因转染可抑制CNE2细胞在裸鼠体内的生长。
