Barrera C N, Mazzolli A B, Mancini R E
Fertil Steril. 1976 Jan;27(1):21-7.
Classic experimental immunologic orchitis was induced in guinea pigs by injection of homologous testis homogenate in complete Freund's adjuvant. Blood samples were obtained periodically for 45 days and the animals were hemicastrated on the 8th day after sensitization. By the 8th day, cytophilic activity was found in the serum simultaneously with several foci or orchitis. Significant levels of this activity were found until day 29, but it was absent in animals sensitized with an unrelated antigen or with complete adjuvant alone. Low and transitory levels were detected in some guinea pigs when antigen was administered with incomplete Freund's adjuvant. It is concluded that cytophilic activity is organ-specific and requires complete Freund's adjuvant. Possible implications in experimental immunologic orchitis are discussed.
通过在完全弗氏佐剂中注射同种睾丸匀浆，在豚鼠中诱发经典的实验性免疫性睾丸炎。在45天内定期采集血样，致敏后第8天对动物进行半阉割。到第8天，在血清中发现嗜细胞活性，同时伴有多个睾丸炎病灶。直到第29天发现这种活性的显著水平，但在用无关抗原或单独用完全佐剂致敏的动物中不存在。当抗原与不完全弗氏佐剂一起给药时，在一些豚鼠中检测到低水平且短暂的活性。得出结论，嗜细胞活性是器官特异性的，并且需要完全弗氏佐剂。讨论了在实验性免疫性睾丸炎中的可能影响。
