Muir V Y, Turk J L, Hanley H G
Clin Exp Immunol. 1976 Apr;24(1):72-80.
Groups of male and female guinea-pigs were immunized with homologous epdidymal sperm in Freund's complete adjuvant (FCA) and skin tested at weekly intervals with a heat-treated extract of the sperm or with PPD. In females, the skin response to both antigens was similar to that to any standard protein antigen. In males, the response to sperm extract varied with the induced auto-immune orchitis, reaching a maximum immediately after testis lesion was most severe and as recovery was beginning (shown histologically): the response to PPD was decreased at 1 week after immunization, but subsequently was similar to that in females. Skin tests on guinea-pigs 8 months after bilateral vasectomy (when both ends of vasa were ligated), showed no evidence of delayed hypersensitivity to sperm, although there was marked histological evidence of reduced spermatognesis, due to back pressure atrophy.
将雄性和雌性豚鼠分组，用弗氏完全佐剂（FCA）中的同源附睾精子进行免疫，并每周用热处理的精子提取物或PPD进行皮肤试验。在雌性中，对两种抗原的皮肤反应与对任何标准蛋白质抗原的反应相似。在雄性中，对精子提取物的反应随诱导的自身免疫性睾丸炎而变化，在睾丸病变最严重且恢复开始时（组织学显示）立即达到最大值：对PPD的反应在免疫后1周降低，但随后与雌性相似。对双侧输精管结扎8个月后的豚鼠进行皮肤试验（输精管两端均结扎），未显示对精子有迟发型超敏反应的证据，尽管由于反压萎缩有明显的组织学证据表明精子发生减少。
