Anderson C H, Schwartz N B, Nequin L G, Ely C A
Fertil Steril. 1976 Jan;27(1):47-58.
Neonatal rats secrete considerable amounts of luteinizing hormone (LH) and follicle-stimulating hormone (FSH) between 5 and 15 days of age. Antisera to LH and FSH were injected during this 11-day period in an attempt to study the importance of gonadotropins for functional development of the gonads. Autopsies at 15 days of age revealed normal ovarian follicular histology after either antiserum treatment, but antiserum to LH significantly retarded differentiation of interstitial tissue. Uterine weights were increased, and some of the serum samples showed elevated FSH levels following the antiserum treatments. The testes of antiserum-treated rats were heavier at 15 days of age, while accessory sex organ weights were unchanged. Vaginal opening after antiserum treatment was more variable than in controls, but tended to be earlier. The first appearance of penile serum was not significantly changed. Both males and females were fertile as adults. Important limitations to the use of chronic antiserum injections were found in immature rats. The antisera were present for at least 34 days after injection. The ways in which this pool of antiserum might have influenced the results are discussed.
新生大鼠在5至15日龄期间分泌大量促黄体生成素（LH）和促卵泡生成素（FSH）。在此11天期间注射LH和FSH抗血清，试图研究促性腺激素对性腺功能发育的重要性。15日龄时进行尸检发现，两种抗血清处理后卵巢卵泡组织学均正常，但LH抗血清显著延缓了间质组织的分化。抗血清处理后子宫重量增加，部分血清样本FSH水平升高。抗血清处理的大鼠在15日龄时睾丸较重，而附属生殖器官重量未变。抗血清处理后的阴道开口比对照组更具变异性，但往往更早出现。阴茎血清的首次出现无显著变化。成年后雄性和雌性均具有生育能力。在未成熟大鼠中发现了长期注射抗血清使用的重要局限性。注射后抗血清至少存在34天。讨论了这一抗血清库可能影响结果的方式。
