Joung K-B, Côté J-C
Research Centre, Agriculture and Agri-Food Canada, St-Jean-sur-Richelieu, Quebec, Canada.
J Appl Microbiol. 2002;93(6):1075-82. doi: 10.1046/j.1365-2672.2002.01788.x.
To assess the congruence between two earlier independent phylogenetic studies on Bacillus thuringiensis strains and on Bacillus-related species and the single, all-encompassing, phylogenetic tree presented here inferred from the combination of the two earlier datasets.
A dendrogram was constructed using a combination of the data from our previous studies on 16S rRNA gene fingerprinting of 86 B. thuringiensis strains and of 77 species of Bacillus and related taxa. It revealed that all B. thuringiensis strains were clustered together in four distinct groups at a DNA similarity rate of 93%, except two serovars, bolivia and finitimus. Four bacilli species, Paenibacillus alvei, P. azotofixans, B. lentus and B. licheniformis, share a DNA similarity rate of 92% with Bt Group IV.
Most, but not all, B. thuringiensis strains could be grouped together based on the DNA similarity rate. They were also very close to some other bacilli species.
The combined phylogenetic study presented here, inferred from 16S rRNA gene restriction fragment length polymorphism, is congruent with two earlier independent phylogenetic studies, one on B. thuringiensis and the other on bacilli species.
评估两项早期关于苏云金芽孢杆菌菌株和芽孢杆菌相关物种的独立系统发育研究，与本文根据这两个早期数据集合并得出的单一、全面的系统发育树之间的一致性。
利用我们之前对86株苏云金芽孢杆菌菌株以及77种芽孢杆菌和相关分类群的16S rRNA基因指纹图谱研究的数据构建了一个树状图。结果显示，除了玻利维亚和有限型这两个血清型外，所有苏云金芽孢杆菌菌株在DNA相似率为93%时聚为四个不同的组。四种芽孢杆菌，即蜂房芽孢杆菌、固氮芽孢杆菌、迟缓芽孢杆菌和地衣芽孢杆菌，与苏云金芽孢杆菌第四组的DNA相似率为92%。
大多数但并非所有的苏云金芽孢杆菌菌株可以根据DNA相似率归为一组。它们也与其他一些芽孢杆菌物种非常接近。
本文通过16S rRNA基因限制性片段长度多态性推断出的综合系统发育研究，与两项早期独立的系统发育研究一致，一项是关于苏云金芽孢杆菌，另一项是关于芽孢杆菌物种。
