Oakey H J, Cullen B R, Owens L
Department of Microbiology and Immunology, James Cook University, Townsville, Queensland 4811, Australia.
J Appl Microbiol. 2002;93(6):1089-98. doi: 10.1046/j.1365-2672.2002.01776.x.
To determine the complete nucleotide sequence of the bacteriophage VHML and establish a hypothesis for the virulence conversion caused by VHML infection of Vibrio harveyi.
The complete nucleotide sequence of VHML was determined (43,193 bp) and used to identify putative genes. The translated products of these genes were compared with reported sequences to assign hypothetical functions. All anticipated structural genes and putative genes for lysogeny were identified. In addition, we found a complete N6-adenine methyltransferase (Dam) gene that appeared to have an essential site for ADP-ribosylating toxins at the C-terminal of the translated product.
Virulence conversion of V. harveyi by VHML may be associated with Dam transcriptional regulation. The Dam gene may also encode for a toxin component similar to ADP-ribosylating toxins.
This manuscript lays the foundation for understanding the virulence of toxin-producing V. harveyi. Further research into aspects discussed here will lead to a greater comprehension regarding the invertebrate disease vibriosis and its control in the farming of these animals.
确定噬菌体VHML的完整核苷酸序列，并建立关于哈维氏弧菌受VHML感染导致毒力转换的假说。
测定了VHML的完整核苷酸序列（43193 bp），并用于鉴定推定基因。将这些基因的翻译产物与已报道的序列进行比较以赋予假定功能。鉴定出了所有预期的结构基因和溶原性推定基因。此外，我们发现了一个完整的N6-腺嘌呤甲基转移酶（Dam）基因，其翻译产物的C末端似乎具有ADP-核糖基化毒素的必需位点。
VHML导致哈维氏弧菌的毒力转换可能与Dam转录调控有关。Dam基因也可能编码一种类似于ADP-核糖基化毒素的毒素成分。
本手稿为理解产毒素哈维氏弧菌的毒力奠定了基础。对本文所讨论方面的进一步研究将有助于更深入地理解无脊椎动物疾病弧菌病及其在这些动物养殖中的控制。
