Rosenow M A, Magee C L, Williams J C, Allen J P
Department of Chemistry and Biochemistry, Arizona State University, Tempe, AZ 85287-1604, USA.
Acta Crystallogr D Biol Crystallogr. 2002 Dec;58(Pt 12):2076-81. doi: 10.1107/s0907444902016736. Epub 2002 Nov 23.
The relationship between the effect of detergents and amphiphiles on protein solubility and their use in crystallization solutions was examined for the reaction center from Rhodobacter sphaeroides. Measurement by a centrifugation assay of the solubility of the reaction center as a function of ionic strength revealed dramatic differences in the intrinsic solubility at zero ionic strength in the presence of various detergents and amphiphiles. High protein-solubility values were found for beta-octyl glucoside and for lauryldimethylamine-N-oxide with heptanetriol. The solubility differences are interpreted in terms of fundamental properties such as the polarity of the detergent molecules. Conditions that resulted in high protein solubility correspond to conditions that have been shown to be successful for crystallization of the reaction center. These results suggest that crystallization is favored for detergents and amphiphiles that optimize the solubility of integral membrane proteins.
研究了去污剂和两亲分子对球形红杆菌反应中心蛋白质溶解度的影响及其在结晶溶液中的应用。通过离心测定法测量反应中心溶解度随离子强度的变化，结果显示在各种去污剂和两亲分子存在下，零离子强度时的固有溶解度存在显著差异。发现β-辛基葡萄糖苷以及月桂基二甲基氧化胺与庚三醇组合具有较高的蛋白质溶解度值。根据去污剂分子的极性等基本性质来解释溶解度差异。导致高蛋白溶解度的条件与已证明对反应中心结晶成功的条件相对应。这些结果表明，对于优化整合膜蛋白溶解度的去污剂和两亲分子，有利于结晶。
