Marques R G, Petroianu A, Coelho J M C O, Portela M C
Laboratory of Experimental Surgery, Department of General Surgery, Medical Sciences School, Rio de Janeiro State University, Rua Clóvis Salgado 280/104, Recreio, Rio de Janeiro, CEP 22795-230, RJ, Brazil.
Ann Hematol. 2002 Nov;81(11):622-6. doi: 10.1007/s00277-002-0564-2. Epub 2002 Nov 9.
Splenic autotransplantation seems to be the only alternative for preservation of splenic tissue after total splenectomy. This work was carried out to analyze the morphologic regeneration of autotransplanted splenic tissue in Wistar rats and to determine the bacterial phagocytic function of their macrophages. We utilized an experimental model including young and adult rats, of both sexes, submitted to total splenectomy combined with autotransplantation in the greater omentum of slices of the whole mass of spleen. Sixteen weeks later animals were intravenously inoculated with a suspension of Escherichia coli AB1157. There was regeneration of autotransplanted splenic tissue in all animals. A similar morphological aspect among all animals was observed, with splenic tissue showing red and white pulps with a moderate architectural disarrangement. Macrophages containing bacterial aggregates were observed, as well as macrophages with hemosiderin pigments inside the cytoplasm. Blood vessels showed preserved walls, with no signs of vasculitis or thrombosis. The present results suggest that splenic autotransplants in the greater omentum of the rat acquire the macro- and microscopic architecture of a normal spleen, with reduced dimensions, and preserve bacterial phagocyte function.
脾自体移植似乎是全脾切除术后保留脾组织的唯一选择。本研究旨在分析 Wistar 大鼠自体移植脾组织的形态学再生情况，并确定其巨噬细胞的细菌吞噬功能。我们采用了一种实验模型，包括年轻和成年大鼠，雌雄皆有，进行全脾切除并将整个脾脏切片自体移植到大网膜中。16 周后，给动物静脉注射大肠杆菌 AB1157 悬液。所有动物的自体移植脾组织均有再生。观察到所有动物的形态相似，脾组织呈现红髓和白髓，结构有中度紊乱。观察到含有细菌聚集体的巨噬细胞，以及细胞质内含有含铁血黄素色素的巨噬细胞。血管壁保存完好，无血管炎或血栓形成迹象。目前的结果表明，大鼠大网膜中的脾自体移植获得了尺寸减小的正常脾脏的宏观和微观结构，并保留了细菌吞噬功能。
