Mauvieux L, Leymarie V, Helias C, Perrusson N, Falkenrodt A, Lioure B, Lutz P, Lessard M
Laboratoire d'Hématologie, Hôpitaux Universitaires de Strasbourg, Strasbourg, France.
Leukemia. 2002 Dec;16(12):2417-22. doi: 10.1038/sj.leu.2402709.
The orphan homeobox gene HOX11L2 was previously found to be transcriptionally activated as a result of the t(5;14)(q35;q32) translocation in three T-ALL cases. We now tested by RT-PCR Hox11L2 expression in 23 consecutive cases of T-ALL (15 children aged 0.8-14 years, eight adults aged 17-55 years) and as control 13 B-ALL patients from a single institution. Hox11L2 expression was undetectable in all patients with B-ALL, nor in adults with T-ALL. Nine children (60% of the cases), all boys, expressed Hox11L2. Blast cells from most of the latter patients carried surface CD1a, CD10 and not CD34 antigens, in contrast to the other children. FISH, M-FISH and IPM-FISH analysis failed to detect a t(5;14)(q35;q32) in one of them, which suggests a possible distinct genetic mechanism in Hox11L2 expression induction. Hence, Hox11L2 expression seems to be the most frequent abnormality in childhood T-ALL to date, comparable to the t(12;21) in child B-ALL.
此前发现，孤儿同源盒基因HOX11L2在3例T-ALL病例中因t(5;14)(q35;q32)易位而转录激活。我们现在通过RT-PCR检测了23例连续的T-ALL病例（15名年龄在0.8至14岁的儿童，8名年龄在17至55岁的成人）以及来自单一机构的13例B-ALL患者作为对照中的Hox11L2表达情况。在所有B-ALL患者以及成人T-ALL患者中均未检测到Hox11L2表达。9名儿童（占病例的60%），均为男孩，表达Hox11L2。与其他儿童不同，大多数后一组患者的原始细胞携带表面CD1a、CD10抗原而不携带CD34抗原。其中1例患者的FISH、M-FISH和IPM-FISH分析未能检测到t(5;14)(q35;q32)，这表明Hox11L2表达诱导可能存在不同的遗传机制。因此，Hox11L2表达似乎是迄今为止儿童T-ALL中最常见的异常情况，与儿童B-ALL中的t(12;21)相当。
