Fukui Shinji, Otani Naoki, Nawashiro Hiroshi, Yano Akiko, Nomura Namiko, Miyazawa Takahito, Ohnuki Akira, Tsuzuki Nobusuke, Katoh Hiroshi, Ishihara Shoichiro, Shima Katsuji
Department of Neurosurgery, National Defense Medical College, 3-2 Namiki, Tokorozawa, Saitama 359-8513, Japan.
Brain Tumor Pathol. 2002;19(1):23-9. doi: 10.1007/BF02482452.
The aim of this study was to assess the subcellular localization of basic fibroblast growth factor (bFGF) and fibroblast growth factor receptor 1 (FGFR1) in pituitary adenomas. We studied 61 patients who had primary pituitary adenomas and underwent operation. The immunohistochemistry for bFGF, FGFR1, and MIB-1 was examined in paraffin-embedded tissues. The bFGF immunoreactivity in the nucleus was recorded as the bFGF nuclear index, which was calculated as the percentage of tumor cells with the bFGF immunoreactivity in the nuclei when more than 1000 tumor cells were examined. Recurrent adenomas were found in 7 patients during follow-up periods ranging from 8 to 134 months (mean, 57.2). The recurrent adenomas had significantly larger mean bFGF nuclear indices (74.8 +/- 28.8%) than the nonrecurrent adenomas (25.4 +/- 32.1%, P = 0.0003). The bFGF nuclear index also correlated significantly with the maximum tumor diameters and the invasiveness to the cavernous sinuses (Knosp grade) in the adenomas. The cytoplasmic FGFR1 immunoreactivity was inversely correlated (P < 0.02) with maximum tumor diameter. Neither cytoplasmic bFGF, cytoplasmic FGFR1, nor MIB-1 staining index showed any relationship with the recurrence of pituitary adenomas. These findings suggest that the nuclear accumulation of bFGF plays an important role in the progression of pituitary adenomas without its receptors.
本研究的目的是评估碱性成纤维细胞生长因子（bFGF）和成纤维细胞生长因子受体1（FGFR1）在垂体腺瘤中的亚细胞定位。我们研究了61例患有原发性垂体腺瘤并接受手术的患者。在石蜡包埋组织中检测了bFGF、FGFR1和MIB-1的免疫组织化学。细胞核中的bFGF免疫反应性被记录为bFGF核指数，当检查超过1000个肿瘤细胞时，它被计算为细胞核中具有bFGF免疫反应性的肿瘤细胞的百分比。在8至134个月（平均57.2个月）的随访期内，7例患者发现复发性腺瘤。复发性腺瘤的平均bFGF核指数（74.8±28.8%）显著高于非复发性腺瘤（25.4±32.1%，P = 0.0003）。bFGF核指数还与腺瘤的最大肿瘤直径以及对海绵窦的侵袭性（Knosp分级）显著相关。细胞质FGFR1免疫反应性与最大肿瘤直径呈负相关（P < 0.02）。细胞质bFGF、细胞质FGFR1或MIB-1染色指数均与垂体腺瘤的复发无关。这些发现表明，bFGF的核积累在无其受体的垂体腺瘤进展中起重要作用。
