Momol M T, Momol E A, Lamboy W F, Norelli J L, Beer S V, Aldwinckle H S
Department of Plant Pathology, Cornell University, Geneva, NY 14456, USA.
J Appl Microbiol. 1997 Mar;82(3):389-98. doi: 10.1046/j.1365-2672.1997.00377.x.
The genetic diversity among 16 strains of Erwinia amylovora, chosen to represent different host plant origins and geographical regions, was investigated by RAPD analysis. One strain of Erwinia herbicola and one of Agrobacterium vitis were used as outgroups. Ninety-eight different RAPD fragments were produced by polymerase chain reaction amplification with six different 10-mer primers. RAPD banding profiles were found that enabled the Erw. amylovora strains to be distinguished from one another. Cluster analysis based on the number of RAPD fragments shared between strains showed that strains of Erw. amylovora isolated from subfamily Pomoideae formed a single group, whereas two strains from Rubus (subfamily Rosoideae) formed a second group. Two strains isolated from Asian pear on Hokkaido, Japan, formed a third group. Sets of RAPD fragments were identified that enabled each of the two host-range groups and one geographical region (Hokkaido) of Erw. amylovora strains to be unambiguously distinguished from one another and from the outgroups. This study shows that strains of Erw. amylovora exhibit genetic diversity detectable by RAPD analysis, and that molecular and statistical analysis of RAPD fragments can be used both to distinguish between strains and to determine relatedness between them.
通过随机扩增多态性DNA（RAPD）分析，对16株梨火疫病菌（Erwinia amylovora）的遗传多样性进行了研究，这些菌株代表了不同的寄主植物来源和地理区域。选用了1株草生欧文氏菌（Erwinia herbicola）和1株葡萄土壤杆菌（Agrobacterium vitis）作为外群。使用6种不同的10聚体引物通过聚合酶链反应扩增产生了98个不同的RAPD片段。发现RAPD条带图谱能够区分梨火疫病菌株。基于菌株间共享的RAPD片段数量进行的聚类分析表明，从苹果亚科分离的梨火疫病菌株形成一个单一组，而从悬钩子属（蔷薇亚科）分离的2个菌株形成第二组。从日本北海道亚洲梨上分离的2个菌株形成第三组。鉴定出了几组RAPD片段，能够明确区分梨火疫病菌株的两个寄主范围组和一个地理区域（北海道）的菌株以及外群。本研究表明，梨火疫病菌株表现出可通过RAPD分析检测到的遗传多样性，并且对RAPD片段的分子和统计分析可用于区分菌株并确定它们之间的亲缘关系。
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