Matsumoto Yukihiro, Takahashi Masaaki, Chikuda Makoto, Arai Kiyomi
Department of Ophthalmology, Koshigaya Hospital, Dokkyo University School of Medicine, Saitama, Japan.
Jpn J Ophthalmol. 2002 Sep-Oct;46(5):510-7. doi: 10.1016/s0021-5155(02)00537-3.
To determine the levels of pyridinoline and deoxypyridinoline, two mature enzymatic cross-links, and pentosidine, an advanced glycation end product (AGE) cross-link, in the human vitreous, and to investigate the correlations among the cross-links and the effects of aging and diabetes mellitus (DM) on the levels of cross-links.
Forty-five vitreous samples were collected from 32 patients (32 eyes) undergoing vitrectomy for diabetic retinopathy (DM group) and from 13 patients (13 eyes) (control group) who were age- and sex-matched patients with idiopathic macular hole or epiretinal membrane with no systemic conditions. The levels of the cross-links were determined using high-performance liquid chromatography after acid hydrolysis and pretreatment with SP-Sephadex.
The levels of pentosidine, pyridinoline, and deoxypyridinoline were 27.3 +/- 23.1 (mean +/- SD) pmol/mL (detectable in 45 of 45 specimens), 79.0 +/- 40.2 ng/mL (43 of 45 specimens), and 54.0 +/- 9.5 (32 of 45 specimens) ng/mL, respectively. When the vitreous samples from the DM and the control groups were compared, a significant difference (P <.05) was found in the pentosidine level but not in the levels of pyridinoline or deoxypyridinoline. No significant correlations were found between age and the cross-links. Significant correlations (P <.01) were found among the cross-links.
The results indicate that mature cross-link substances exist in the human vitreous. The results also suggest that glycation may occur in the vitreous after mature cross-links form and result in the formation of AGE cross-links. In human vitreous from patients with DM, increased levels of AGE cross-links may stabilize the formation of mature cross-links, but they did not increase the mature cross-links.
测定人体玻璃体中两种成熟的酶促交联物吡啶啉和脱氧吡啶啉以及晚期糖基化终产物（AGE）交联物戊糖苷的水平，并研究这些交联物之间的相关性以及衰老和糖尿病（DM）对交联物水平的影响。
从32例因糖尿病性视网膜病变接受玻璃体切除术的患者（DM组，32只眼）和13例年龄及性别匹配、无全身疾病的特发性黄斑裂孔或视网膜前膜患者（对照组，13只眼）中收集45份玻璃体样本。经酸水解并用SP-葡聚糖凝胶预处理后，采用高效液相色谱法测定交联物水平。
戊糖苷、吡啶啉和脱氧吡啶啉的水平分别为27.3±23.1（平均值±标准差）pmol/mL（45份标本中有45份可检测到）、79.0±40.2 ng/mL（45份标本中有43份）和54.0±9.5（45份标本中有32份）ng/mL。比较DM组和对照组的玻璃体样本时，发现戊糖苷水平有显著差异（P<.05），而吡啶啉或脱氧吡啶啉水平无显著差异。未发现年龄与交联物之间有显著相关性。交联物之间存在显著相关性（P<.01）。
结果表明人体玻璃体中存在成熟的交联物质。结果还提示，在成熟交联物形成后，玻璃体中可能发生糖基化并导致AGE交联物的形成。在DM患者的人体玻璃体中，AGE交联物水平升高可能会稳定成熟交联物的形成，但不会增加成熟交联物的含量。
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