Deininger Martin H, Fimmen Birte, Kremsner Peter G, Meyermann Richard, Schluesener Hermann J
Institute of Brain Research, University of Tuebingen, Medical School, Calwer Str. 3, Tübingen, Germany.
J Neuroimmunol. 2002 Oct;131(1-2):216-21. doi: 10.1016/s0165-5728(02)00276-x.
Endostatin is a 20 kDa C-terminal fragment of collagenXVIII that, when added exogenously, inhibits angiogenesis by inducing apoptosis of endothelial cells. In cerebral malaria (CM), blood-brain barrier dysfunction is a hallmark alteration in the formation of edema, inflammation, hemorrhage and Dürck's granulomas that are thought to represent the histopathological basis of neurological impairments observed in CM patients. We now analyzed endostatin/collagenXVIII expression in brains of seven patients who died with CM and in seven control patients by immunohistochemistry double-labeling experiments. Endostatin/collagenXVIII immunoreactive macrophages/microglial cells accumulated predominantly in Dürck's granulomas. Some immunoreactivity was observed in macrophages located in cerebral capillaries with deposition of malarial pigment and sequestration, but almost no immunoreactivity was detected in ring hemorrhages. Focal accumulation of endostatin/collagenXVIII in granulomas but not in ring hemorrhages of CM brains suggests a novel process that is involved in the destruction of endothelial cells at the time of Dürck's granuloma formation.
内皮抑素是胶原蛋白XVIII的一个20 kDa的C末端片段，当外源性添加时，它通过诱导内皮细胞凋亡来抑制血管生成。在脑型疟疾（CM）中，血脑屏障功能障碍是水肿、炎症、出血和迪尔克肉芽肿形成的标志性改变，这些被认为是CM患者神经功能损害的组织病理学基础。我们现在通过免疫组织化学双标记实验分析了7例死于CM的患者和7例对照患者大脑中的内皮抑素/胶原蛋白XVIII表达。内皮抑素/胶原蛋白XVIII免疫反应性巨噬细胞/小胶质细胞主要聚集在迪尔克肉芽肿中。在脑毛细血管中含有疟色素沉积和红细胞滞留的巨噬细胞中观察到一些免疫反应性，但在环状出血中几乎未检测到免疫反应性。CM脑肉芽肿中内皮抑素/胶原蛋白XVIII的局灶性聚集而非环状出血中的聚集表明，在迪尔克肉芽肿形成时，这是一个参与内皮细胞破坏的新过程。
