Sodian Ralf, Lemke Thees, Fritsche Clemens, Hoerstrup Simon P, Fu Ping, Potapov Evgenij V, Hausmann Harald, Hetzer Roland
Department of Thoracic and Cardiovascular Surgery, Laboratory for Tissue Engineering, German Heart Institute Berlin, Augustenburger Platz 1, 13353 Berlin, Germany.
Tissue Eng. 2002 Oct;8(5):863-70. doi: 10.1089/10763270260424222.
One approach to the tissue engineering of vascular structures is to develop in vitro conditions in order ultimately to fabricate functional vascular tissues before final implantation. In our experiment, we aimed to develop a new combined cell seeding and perfusion system that provides sterile conditions during cell seeding and biomechanical stimuli in order to fabricate autologous human vascular tissue in vitro. The cell seeding and perfusion system is made of Plexiglas and is completely transparent (Berlin Heart, Berlin, Germany; University Hospital Benjamin Franklin, Berlin, Germany). The whole system consists of a cell seeding chamber that can be incorporated into the perfusion system and an air-driven respirator pump connected to the bioreactor. The cell culture medium continuously circulates through a closed-loop system. We thus developed a cell seeding device for static and dynamic seeding of vascular cells onto a polymeric vascular scaffold and a closed-loop perfused bioreactor for long-term vascular conditioning. The cell seeding chamber can be easily connected to the bioreactor, which combines continuous, pulsatile perfusion and mechanical stimulation to the tissue-engineered conduit. Adjusting the stroke volume, the stroke rate, and the inspiration/expiration time of the ventilator allows various pulsatile flows and different levels of pressure. The whole system is a highly isolated cell culture setting, which provides a high level of sterility and a gas supply and fits into a standard humidified incubator. The device can be sterilized by ethylene oxide and assembled with a standard screwdriver. Our newly developed combination of a cell seeding and conditioning device provides sterile conditions and biodynamic stimuli for controlled tissue development and in vitro conditioning of an autologous tissue-engineered vessel.
血管结构组织工程的一种方法是在体外创造条件，以便最终在最终植入前制造出功能性血管组织。在我们的实验中，我们旨在开发一种新的细胞接种与灌注系统，该系统在细胞接种过程中提供无菌条件以及生物力学刺激，以便在体外制造自体人类血管组织。细胞接种与灌注系统由有机玻璃制成，完全透明（德国柏林的柏林心脏公司；德国柏林的本杰明·富兰克林大学医院）。整个系统由一个可并入灌注系统的细胞接种室和一个连接到生物反应器的气动呼吸泵组成。细胞培养基通过一个闭环系统持续循环。因此，我们开发了一种用于将血管细胞静态和动态接种到聚合物血管支架上的细胞接种装置，以及一个用于长期血管调节的闭环灌注生物反应器。细胞接种室可以很容易地连接到生物反应器上，该生物反应器将连续、脉动灌注和对组织工程管道的机械刺激结合在一起。调节呼吸机的冲程容积、冲程速率和吸气/呼气时间可实现各种脉动流和不同水平的压力。整个系统是一个高度隔离的细胞培养环境，可提供高水平的无菌条件和气体供应，并可放入标准的加湿培养箱中。该装置可用环氧乙烷消毒，并用标准螺丝刀组装。我们新开发的细胞接种与调节装置组合为自体组织工程血管的受控组织发育和体外调节提供了无菌条件和生物动力学刺激。
