Nagamanju P, Hansen I A, Burmester T, Meyer S R, Scheller K, Dutta-Gupta A
Department of Animal Sciences, School of Life Sciences, University of Hyderabad, 500 046, Hyderabad, India.
Insect Biochem Mol Biol. 2003 Jan;33(1):73-80. doi: 10.1016/s0965-1748(02)00178-9.
Three distinct types of storage hexamerins are expressed in the "last-instar" larvae of the rice moth, Corcyra cephalonica. A cDNA expression library was constructed from fat body-RNA and screened with a polyclonal antibody raised against purified hexamerin (SP2) of Corcyra cephalonica. Two slightly different "full-length" hexamerin cDNA clones (Hex2a and Hex2b) were isolated and sequenced. Both include open reading frames of 2109 bp which are translated into polypeptides of 703 amino acids with 92.5% identity. Signal peptides of 19 amino acids are present at the N-termini. The 684 amino acids native proteins have a high content of aryl groups (17.6%). According to both the criteria for amino acid composition and the phylogenetic analysis, Hex2a and Hex2b belong to the lepidopteran arylphorins. Northern blot studies revealed that the Hex2 genes are species- and tissue-specifically expressed in fat body cells of "last-instar" (= 5th) larvae.
在米蛾（Corcyra cephalonica）的“末龄”幼虫中表达了三种不同类型的储存六聚体蛋白。从脂肪体RNA构建了一个cDNA表达文库，并用针对纯化的米蛾六聚体蛋白（SP2）产生的多克隆抗体进行筛选。分离并测序了两个略有不同的“全长”六聚体蛋白cDNA克隆（Hex2a和Hex2b）。两者都包含2109 bp的开放阅读框，可翻译成703个氨基酸的多肽，同一性为92.5%。在N端存在19个氨基酸的信号肽。684个氨基酸的天然蛋白具有高含量的芳基（17.6%）。根据氨基酸组成标准和系统发育分析，Hex2a和Hex2b属于鳞翅目芳基转运蛋白。Northern印迹研究表明，Hex2基因在“末龄”（=第5龄）幼虫的脂肪体细胞中进行物种特异性和组织特异性表达。
