Department of Animal Sciences, School of Life Sciences, University of Hyderabad, Gachibowli, Hyderabad, Andhra Pradesh, India.
J Insect Physiol. 2010 Sep;56(9):1071-7. doi: 10.1016/j.jinsphys.2010.02.017. Epub 2010 Mar 23.
Selective receptor mediated uptake is a widely prevalent mechanism in insects by which important macromolecules are acquired. Among the various proteins sequestered by the insect fat body, the larval hexamerins form the major group. In the present work full length cDNA (2.6kb) of hexamerin receptor with an ORF of 2.4kb was cloned from the larval fat body of rice moth, Corcyra cephalonica. This was followed by the recombinant expression of truncated N-terminal sequence of putative hexamerin receptor and the confirmation of the expressed recombinant protein as the truncated hexamerin receptor by ligand blot analysis. Apart from this we also analyzed other hexamerin sequestering tissues like salivary gland, male accessory reproductive gland and ovary for the presence of hexamerin receptor. We found that the receptor in these tissues was similar in size and mode of activation to that of fat body hexamerin receptor, thus cementing the fact that identical hexamerin receptors are present in all the hexamerin sequestering tissues in the rice moth.
选择性受体介导的摄取是昆虫中广泛存在的一种机制，通过这种机制可以获取重要的大分子物质。在昆虫脂肪体中摄取的各种蛋白质中，幼虫六聚体蛋白形成了主要的群体。在本工作中，我们从水稻螟虫（Corcyra cephalonica）的幼虫脂肪体中克隆了全长 cDNA（2.6kb），其开放阅读框为 2.4kb。接下来，我们对假定的六聚体受体的截断 N 端序列进行了重组表达，并通过配体印迹分析证实表达的重组蛋白为截断的六聚体受体。除此之外，我们还分析了唾液腺、雄性附属性生殖腺和卵巢等其他六聚体摄取组织中是否存在六聚体受体。我们发现，这些组织中的受体在大小和激活方式上与脂肪体六聚体受体相似，从而证实了在水稻螟虫的所有六聚体摄取组织中都存在相同的六聚体受体这一事实。
