Wang Zhen-Guo, Ye Richard D
Department of Pharmacology, College of Medicine, University of Illinois, Chicago, IL 60612, USA.
Gene. 2002 Oct 16;299(1-2):57-63. doi: 10.1016/s0378-1119(02)01012-0.
Formyl peptide receptors are G-protein-coupled, seven-transmembrane domain receptors originally identified in leukocytes. Ligands for this class of receptors include the chemotactic peptide fMet-Leu-Phe, lipoxin A(4), serum amyloid A and beta-amyloid peptides. The formyl peptide receptor gene family contains three members in human and six members in mouse. By screening a mouse genomic library, we isolated two novel genes that were provisionally named Fpr-rs6 and Fpr-rs7. They encode putative seven-transmembrane domain proteins of 339 and 338 residues, respectively, and share between them 94% amino acid identity. The predicted amino acid sequences of Fpr-rs6 and Fpr-rs7 are 53-74% identical to other mouse formyl peptide receptors. The transcript of Fpr-rs6 is found in brain, spleen and skeletal muscle, and at high level in testis. The Fpr-rs7 transcript is more widely expressed in heart, liver, lung, spleen, smooth muscle and pancreas. Our data suggest that the expression of Fpr-rs6 and Fpr-rs7 is differentially regulated in mouse despite their high sequence homology.
甲酰肽受体是最初在白细胞中发现的与G蛋白偶联的七跨膜结构域受体。这类受体的配体包括趋化肽fMet-Leu-Phe、脂氧素A(4)、血清淀粉样蛋白A和β-淀粉样肽。甲酰肽受体基因家族在人类中包含三个成员，在小鼠中包含六个成员。通过筛选小鼠基因组文库，我们分离出两个新基因，暂命名为Fpr-rs6和Fpr-rs7。它们分别编码由339和338个残基组成的推定七跨膜结构域蛋白，二者之间具有94%的氨基酸同一性。Fpr-rs6和Fpr-rs7的预测氨基酸序列与其他小鼠甲酰肽受体具有53-74%的同一性。Fpr-rs6的转录本在脑、脾和骨骼肌中被发现，在睾丸中高水平表达。Fpr-rs7转录本在心脏、肝脏、肺、脾、平滑肌和胰腺中表达更为广泛。我们的数据表明，尽管Fpr-rs6和Fpr-rs7具有高度的序列同源性，但它们在小鼠中的表达受到不同的调控。
