Burton Edward A, Bai Qing, Goins William F, Glorioso Joseph C
Department of Molecular Genetics and Biochemistry, School of Medicine, University of Pittsburgh, Pittsburgh, PA 15261, USA.
Curr Opin Biotechnol. 2002 Oct;13(5):424-8. doi: 10.1016/s0958-1669(02)00359-2.
Herpes simplex virus (HSV) may be engineered to produce flexible and efficient gene delivery vectors. Recent advances in vector design and production have built on increasing understanding of the basic biology of HSV to minimise vector toxicity and exploit viral features that give rise to lifelong latent infection in the nervous system. In addition, the emerging picture of viral cell entry has allowed early steps to be taken towards targeting viral entry to predetermined cellular subsets. Recent work has established sound principles for the straightforward production of large-scale pure preparations of vector stocks for clinical applications.
单纯疱疹病毒（HSV）可被改造以产生灵活且高效的基因递送载体。基于对HSV基础生物学的深入理解，载体设计和生产方面的最新进展致力于将载体毒性降至最低，并利用病毒在神经系统中引发终身潜伏感染的特性。此外，病毒进入细胞的新情况使得能够朝着将病毒进入靶向预定细胞亚群的方向尽早采取措施。最近的工作已经确立了为临床应用直接生产大规模纯载体储备制剂的可靠原则。
