现代生物医学科学中的蛋白质组学技术。

Proteomic technologies in modern biomedical science.

作者信息

Govorun V M, Archakov A I

机构信息

Orekhovich Institute of Biomedical Chemistry, Russian Academy of Medical Sciences, Moscow, 119992 Russia.

出版信息

Biochemistry (Mosc). 2002 Oct;67(10):1109-23. doi: 10.1023/a:1020959106412.

DOI:10.1023/a:1020959106412

PMID:12460109

Abstract

This review highlights modern technologies employed in proteomics. Methods of sample preparations are discussed with special emphasis on the requirements for preparation of biological material, which may seriously influence the results of proteomic studies. Methods of solubilization, electrophoresis, chromatographic protein separation, and visualization of protein spots in gels are described. Modern methods of mass spectrometry used in proteomic studies include combination of protein chips with mass spectrometry. The review also describes approaches of functional proteomics, i.e., interactomics, and also bioinformatic resources used in proteomics for image analysis of 2D-gel-electrophoresis and for identification of protein sequences by mass spectra.

摘要

本综述重点介绍了蛋白质组学中使用的现代技术。讨论了样品制备方法，特别强调了生物材料制备的要求，这可能会严重影响蛋白质组学研究的结果。描述了蛋白质溶解、电泳、色谱蛋白质分离以及凝胶中蛋白质斑点可视化的方法。蛋白质组学研究中使用的现代质谱方法包括蛋白质芯片与质谱联用。本综述还描述了功能蛋白质组学的方法，即相互作用组学，以及蛋白质组学中用于二维凝胶电泳图像分析和通过质谱鉴定蛋白质序列的生物信息学资源。