Cavalcanti Y V N, Leal N C, De Almeida A M P
Universidade Federal Rural de Pernambuco, and Centro de Pesquisas Aggeu Magalhães - FIOCRUZ, Recife (PE), Brazil.
Lett Appl Microbiol. 2002;35(6):543-7. doi: 10.1046/j.1472-765x.2002.01226.x.
To investigate whether modifications in Yersinia pestis isolates from three plague foci from the state of Ceará, Brazil, had occurred over the years as a consequence of genetic adaptation to the environment.
The isolates were studied with respect to susceptibility to antimicrobial drugs, plasmid and protein profiling, pigmentation on Congo red-agar plates, and the presence of some pathogenicity genes using PCR. Most of the expected virulence markers were detected in the cultures examined. There was no evidence of any alteration that could be associated with their origin (patients, rodents and fleas) or period of isolation (1971-1997).
CONCLUSIONS, SIGNIFICANCE AND IMPACT OF THE STUDY: Phenotypic or genotypic changes were not detected in the cultures examined. However, the results obtained will serve as a reference to follow the evolution of Y. pestis in these foci.
调查巴西塞阿拉州三个鼠疫疫源地的鼠疫耶尔森菌分离株多年来是否因对环境的遗传适应而发生了改变。
对分离株进行了抗菌药物敏感性、质粒和蛋白质谱分析、刚果红琼脂平板上的色素沉着以及使用聚合酶链反应检测某些致病基因的研究。在所检测的培养物中检测到了大多数预期的毒力标记。没有证据表明存在任何与它们的来源（患者、啮齿动物和跳蚤）或分离时期（1971 - 1997年）相关的改变。
研究的结论、意义和影响：在所检测的培养物中未检测到表型或基因型变化。然而，所获得的结果将作为追踪这些疫源地鼠疫耶尔森菌进化的参考。
