Sams Reeder L, Couch Letha H, Miller Barbara J, Okerberg Carlin V, Warbritton Alan R, Wamer Wayne G, Beer Janusz Z, Howard Paul C
Division of Biochemical Toxicology, National Center for Toxicology Research, U.S. Food and Drug Administration, Jefferson, Arkansas 72079, USA.
Toxicol Appl Pharmacol. 2002 Nov 1;184(3):136-43. doi: 10.1006/taap.2002.9498.
alpha- and beta-Hydroxy acids have been used extensively in cosmetic and dermatological formulations. At present, there is an inadequate amount of information with which to assess the safety of topical applications of alpha- and beta-hydroxy acids in conjunction with exposure to ultraviolet light. In the present study, we examined changes in the epidermal basal cell proliferation and the edemal response using skin thickness measurements elicited in SKH-1 mice following exposure to simulated solar light (SSL) with or without topical treatment with creams containing alpha- (glycolic) and beta-hydroxy (salicylic) acids. The dose of SSL light required to induce measurable edema (MED(BIOL)) in nai;ve, free-moving SKH-1 mice was determined to be 90 mJ. CIE/cm(2). Pretreating the mice with daily (5 days/week) exposures of 14 mJ. CIE/cm(2) for 6 weeks resulted in a doubling of the MED(BIOL) to 180 mJ. CIE/cm(2). Topical application of control cream (pH 3.5), or creams containing glycolic acid (10%, pH 3.5) or salicylic acid (4%, pH 3.5) for 6 weeks (5 days/week) increased the MED(BIOL) to 137 mJ. CIE/cm(2). Daily treatments with SSL (14 mJ. CIE/cm(2)) and control cream (pH 3.5), glycolic (10%, pH 3.5) or salicylic (4%, pH 3.5) acid-containing creams for 6 weeks (5 days/week) resulted in an MED(BIOL) value of 180 mJ. CIE/cm(2), which was the same as treatment with light alone for 6 weeks. These data indicate that a 6-week treatment of mouse skin with a representative skin cream, with or without representative alpha- and beta-hydroxy acids (glycolic and salicylic acid, respectively), changes the UV light sensitivity; however, treatment with the cream, with or without the acids, does not contribute to the UV sensitivity of mice cotreated with low doses of UV light.
α-羟基酸和β-羟基酸已广泛应用于化妆品和皮肤病学配方中。目前，关于α-羟基酸和β-羟基酸局部应用并暴露于紫外线时安全性的评估信息不足。在本研究中，我们通过测量SKH-1小鼠的皮肤厚度，研究了在有或没有局部涂抹含α-（乙醇酸）和β-羟基（水杨酸）酸乳膏的情况下，暴露于模拟太阳光（SSL）后表皮基底细胞增殖和水肿反应的变化。在未处理、自由活动的SKH-1小鼠中，诱导可测量水肿所需的SSL光剂量（MED(BIOL)）被确定为90 mJ·CIE/cm²。每天（每周5天）以14 mJ·CIE/cm²的剂量对小鼠进行6周的预处理，导致MED(BIOL)增加一倍，达到180 mJ·CIE/cm²。局部涂抹对照乳膏（pH 3.5）、含乙醇酸（10%，pH 3.5）或水杨酸（4%，pH 3.5）的乳膏6周（每周5天），使MED(BIOL)增加到137 mJ·CIE/cm²。每天用SSL（14 mJ·CIE/cm²）和对照乳膏（pH 3.5）、含乙醇酸（10%，pH 3.5）或水杨酸（4%，pH 3.5）的乳膏进行6周（每周5天）的治疗，MED(BIOL)值为180 mJ·CIE/cm²，与仅光照治疗6周相同。这些数据表明，用代表性的护肤霜对小鼠皮肤进行6周治疗，无论是否添加代表性的α-和β-羟基酸（分别为乙醇酸和水杨酸），都会改变紫外线敏感性；然而，无论是否添加酸，使用该乳膏治疗对低剂量紫外线共同处理的小鼠的紫外线敏感性没有影响。
