Brannigan James A, Wilkinson Anthony J
Department of Chemistry, University of York, York YO10 5YW, UK.
Nat Rev Mol Cell Biol. 2002 Dec;3(12):964-70. doi: 10.1038/nrm975.
It is 20 years since site-directed mutagenesis was first used to modify the active site of an enzyme of known structure and mechanism. Since then, this method has contributed far-reaching insights into catalysis, specificity, stability and folding of proteins. Engineered proteins are now being used in industry and for the improved treatment of human disease.
自定点诱变首次用于修饰已知结构和机制的酶的活性位点以来，已经过去了20年。从那时起，这种方法为蛋白质的催化、特异性、稳定性和折叠提供了深远的见解。工程蛋白现在正用于工业和改善人类疾病的治疗。
