Protein engineering 20 years on.
作者信息
Brannigan James A, Wilkinson Anthony J
机构信息
Department of Chemistry, University of York, York YO10 5YW, UK.
出版信息
Nat Rev Mol Cell Biol. 2002 Dec;3(12):964-70. doi: 10.1038/nrm975.
DOI:10.1038/nrm975
PMID:12461562
Abstract
It is 20 years since site-directed mutagenesis was first used to modify the active site of an enzyme of known structure and mechanism. Since then, this method has contributed far-reaching insights into catalysis, specificity, stability and folding of proteins. Engineered proteins are now being used in industry and for the improved treatment of human disease.
摘要
Zotero 插件