Brack Werner, Schirmer Kristin, Kind Tobias, Schrader Steffi, Schüürmann Gerrit
Department of Chemical Ecotoxicology, UFZ Center for Environmental Research Leipzig-Halle, Permoserstrasse 15, 04318 Leipzig, Germany.
Environ Toxicol Chem. 2002 Dec;21(12):2654-62.
On the basis of a new fractionation method combined with in vitro ethoxyresorufin-O-deethylase (EROD) induction in a rainbow trout liver cell line (RTL-W1) and chemical analysis, halogenated aromatic hydrocarbons with dioxin-like activity were identified in a sediment extract from Bitterfeld, Germany. The fractionation method allowed a separation of different nonplanar and coplanar polychlorinated biphenyls (PCBs), polychlorinated naphthalenes (PCNs), dibenzo-p-dioxins (PCDDs), and dibenzofurans (PCDFs) with different degrees of chlorination. The dioxin-like activity at the investigated site could be quantitatively assigned to PCDD/Fs. Both PCBs and PCNs could be excluded as the cause of the measured effects on the basis of the fractionation procedure and bioanalytical results. Thus, the method allowed the chemical analysis to focus on PCDD/Fs, with significant reduction of the analytical expense. The EROD-induction potency of sediment-extract fractions was quantified, and toxicants were confirmed by the application of induction equivalent quantities on the basis of fixed-effect-level concentrations that exhibit 15% of the maximum induction by 2,3,7,8-tetrachlorodibenzo-p-dioxin. This approach was designed to minimize methodological limitations due to superimposing inhibitory effects.
基于一种新的分级分离方法，结合在虹鳟肝细胞系（RTL-W1）中的体外乙氧基异吩恶唑酮-O-脱乙基酶（EROD）诱导以及化学分析，在德国比特费尔德的沉积物提取物中鉴定出了具有二恶英样活性的卤代芳烃。该分级分离方法能够分离不同程度氯化的不同非平面和共平面多氯联苯（PCBs）、多氯萘（PCNs）、二苯并对二恶英（PCDDs）和二苯并呋喃（PCDFs）。研究地点的二恶英样活性可以定量归因于PCDD/Fs。根据分级分离程序和生物分析结果，可以排除PCBs和PCNs是所测效应的原因。因此，该方法使化学分析能够专注于PCDD/Fs，显著降低了分析成本。对沉积物提取物级分的EROD诱导效力进行了定量，并根据表现出2,3,7,8-四氯二苯并对二恶英最大诱导量15%的固定效应水平浓度，通过应用诱导当量来确认有毒物质。这种方法旨在尽量减少由于叠加抑制作用而产生的方法学局限性。
