Burton M D, Papalia L, Eusebius N P, O'Hehir R E, Rolland J M
Department of Pathology and Immunology, Monash University Medical School, Victoria, Australia.
Allergy. 2002 Dec;57(12):1136-44. doi: 10.1034/j.1398-9995.2002.23699.x.
Knowledge of dominant T cell epitopes of major allergens recognized by allergic individuals is required to improve efficacy and safety of allergen immunotherapy. Rye grass pollen (RGP) is the most important source of seasonal aeroallergens in temperate climates and Lol p 1 and Lol p 5 are the two major IgE-reactive allergens. This study aimed to characterize the T cell response to these allergens using a large panel of RGP-sensitive individuals.
Short-term RGP-specific T cell lines (TCL) were generated from 38 RGP-sensitive subjects and stimulated with Lol p 1 and/or Lol p 5 allergens and synthetic 20-mer peptides. Proliferative responses were determined by 3H-thymidine uptake and IL-5 and IFN-gamma in culture supernatants analysed by ELISA.
Of 17 subjects tested for reactivity to both allergens 16 (94%) responded to Lol p 1 and/or Lol p 5, establishing these as major T cell-reactive allergens. Sites of T cell reactivity were spread throughout the allergen molecules but regions of high reactivity were found. For Lol p 1 these spanned residues 19-38, 109-128, 154-173, 190-209, and for Lol p 5 37-56, 100-119, 145-164, 154-173, 190-209, 217-236 and 226-245. IL-5 and IFN-gamma were produced by T cells cultured with proliferation-inducing peptides.
T cell responses to RGP major allergens have been extensively characterized, providing fundamental information for developing T cell-targeted immunotherapy for RGP allergy.
了解过敏个体识别的主要过敏原的显性T细胞表位，对于提高过敏原免疫疗法的疗效和安全性至关重要。黑麦草花粉（RGP）是温带气候中季节性气传过敏原的最重要来源，而Lol p 1和Lol p 5是两种主要的IgE反应性过敏原。本研究旨在使用大量对RGP敏感的个体来表征对这些过敏原的T细胞反应。
从38名对RGP敏感的受试者中产生短期RGP特异性T细胞系，并用Lol p 1和/或Lol p 5过敏原以及合成的20肽进行刺激。通过3H-胸腺嘧啶核苷摄取测定增殖反应，并通过ELISA分析培养上清液中的IL-5和IFN-γ。
在测试对两种过敏原反应性的17名受试者中，16名（94%）对Lol p 1和/或Lol p 5有反应，确定它们为主要的T细胞反应性过敏原。T细胞反应位点遍布过敏原分子，但发现了高反应性区域。对于Lol p 1，这些区域跨越第19 - 38位、第109 - 128位、第154 - 173位、第190 - 209位残基；对于Lol p 5，跨越第37 - 56位、第100 - 119位、第145 - 164位、第154 - 173位、第190 - 209位、第217 - 236位和第226 - 245位残基。用诱导增殖的肽培养的T细胞产生IL-5和IFN-γ。
已广泛表征了对RGP主要过敏原的T细胞反应，为开发针对RGP过敏的T细胞靶向免疫疗法提供了基础信息。
