Blaher B, Suphioglu C, Knox R B, Singh M B, McCluskey J, Rolland J M
Department of Pathology and Immunology, Monash University, Prahran, Victoria, Australia.
J Allergy Clin Immunol. 1996 Jul;98(1):124-32. doi: 10.1016/s0091-6749(96)70234-8.
T-cell recognition of Lol p 9, a major allergen of ryegrass pollen, was investigated by using a T-cell line and T-cell clones generated from the peripheral blood of an atopic donor. The T-cell line reacted with purified Lol p 9, as well as with crude ryegrass pollen extract, but failed to cross-react with Bermuda grass pollen extract. All of six T-cell clones generated from this line proliferated in response to Lol p 9. Epitope mapping was carried out with a panel of 34 overlapping synthetic peptides, which spanned the entire sequence of the Lol p 9 12R isoform. The T-cell line responded to two of the peptides, Lol p 9 (105-116) and Lol p 9 (193-204), whereas reactivity with one or other of these peptides was shown by five T-cell clones. These two peptides contained sequences consistent with motifs previously reported for major histocompatibility complex class II-restricted peptides. HLA antibody blocking studies showed that presentation of peptide Lol p 9 (105-116) to one T-cell clone was HLA-DR-restricted; this clone expressed a T helper cell phenotype (CD3+, CD4+) and the T-cell receptor alpha beta. The identification of immunodominant T-cell epitope(s) on allergens is essential for devising safer and more effective immunotherapy strategies, which can interrupt the chain of events leading to allergic disease.
利用从一名特应性供体的外周血中产生的T细胞系和T细胞克隆，研究了黑麦草花粉主要变应原Lol p 9的T细胞识别情况。该T细胞系与纯化的Lol p 9以及粗制黑麦草花粉提取物发生反应，但与百慕大草花粉提取物无交叉反应。从该细胞系产生的6个T细胞克隆均对Lol p 9产生增殖反应。用一组34个重叠合成肽进行表位作图，这些肽覆盖了Lol p 9 12R异构体的整个序列。该T细胞系对其中两个肽Lol p 9 (105 - 116)和Lol p 9 (193 - 204)有反应，而5个T细胞克隆对其中一个或另一个肽有反应性。这两个肽包含的序列与先前报道的主要组织相容性复合体II类限制性肽的基序一致。HLA抗体阻断研究表明，向一个T细胞克隆呈递肽Lol p 9 (105 - 116)受HLA - DR限制；该克隆表达T辅助细胞表型(CD3 +, CD4 +)和T细胞受体αβ。确定变应原上的免疫显性T细胞表位对于设计更安全、更有效的免疫治疗策略至关重要，这些策略可以中断导致过敏性疾病的一系列事件。
