Doitsidou Maria, Reichman-Fried Michal, Stebler Jürg, Köprunner Marion, Dörries Julia, Meyer Dirk, Esguerra Camila V, Leung TinChung, Raz Erez
Germ Cell Development, Max Planck Institute for Biophysical Chemistry, Am Fassberg 11, 37077, Göttingen, Germany.
Cell. 2002 Nov 27;111(5):647-59. doi: 10.1016/s0092-8674(02)01135-2.
The signals directing primordial germ cell (PGC) migration in vertebrates are largely unknown. We demonstrate that sdf-1 mRNA is expressed in locations where PGCs are found and toward which they migrate in wild-type as well as in mutant embryos in which PGC migration is abnormal. Knocking down SDF-1 or its receptor CXCR4 results in severe defects in PGC migration. Specifically, PGCs that do not receive the SDF-1 signal exhibit lack of directional movement toward their target and arrive at ectopic positions within the embryo. Finally, we show that the PGCs can be attracted toward an ectopic source of the chemokine, strongly suggesting that this molecule provides a key directional cue for the PGCs.
在脊椎动物中，指导原始生殖细胞（PGC）迁移的信号在很大程度上尚不清楚。我们证明，sdf-1 mRNA在野生型以及PGC迁移异常的突变胚胎中PGC所在位置及其迁移方向上均有表达。敲低SDF-1或其受体CXCR4会导致PGC迁移出现严重缺陷。具体而言，未接收到SDF-1信号的PGC表现出缺乏向其靶标的定向移动，并到达胚胎内的异位位置。最后，我们表明PGC可被趋化因子的异位来源所吸引，这强烈表明该分子为PGC提供了关键的定向线索。
