Reichman-Fried Michal, Minina Sofia, Raz Erez
Germ Cell Development, Max Planck Institute for Biophysical Chemistry, Am Fassberg 11, 37077 Göttingen, Germany.
Dev Cell. 2004 Apr;6(4):589-96. doi: 10.1016/s1534-5807(04)00074-7.
Zebrafish primordial germ cells (PGCs) are guided toward their targets by the chemokine SDF-1a. PGCs were followed during three phases of their migration: when migrating as individual cells, while remaining in a clustered configuration, and when moving as a cell cluster within the embryo. We found that individually migrating PGCs alternate between migratory and pausing modes. Pausing intervals are characterized by loss of cell polarity and correlate with subsequent changes in the direction of migration. These properties constitute an intrinsic behavior of PGCs, enabling erasure of prior polarity and re-sampling of the environment. Following migration arrest at a site of high SDF-1a levels, PGCs resume migration as a cluster. The seemingly coordinated cluster migration is a result of single-cell movement in response to local variations in SDF-1a distribution. Together, these behavioral modes allow the cells to arrive at specific destinations with high fidelity and remain at their target site.
斑马鱼原始生殖细胞（PGCs）由趋化因子SDF-1a引导至其目标位置。在PGCs迁移的三个阶段对其进行了追踪：作为单个细胞迁移时、保持聚集状态时以及作为细胞簇在胚胎内移动时。我们发现，单独迁移的PGCs在迁移模式和暂停模式之间交替。暂停间隔的特征是细胞极性丧失，并与随后迁移方向的变化相关。这些特性构成了PGCs的一种内在行为，能够消除先前的极性并对环境进行重新采样。在高SDF-1a水平的位点迁移停滞之后，PGCs作为一个细胞簇恢复迁移。看似协调的细胞簇迁移是单个细胞响应SDF-1a分布的局部变化而移动的结果。总之，这些行为模式使细胞能够高保真地到达特定目的地并停留在其目标位点。
