Baumeister Wolfgang
Department of Structural Biology, Max Planck Institute of Biochemistry, Am Klopferspitz 18a, D-82152 Martinsried, Germany.
Curr Opin Struct Biol. 2002 Oct;12(5):679-84. doi: 10.1016/s0959-440x(02)00378-0.
Electron tomography of thin sections of plastic-embedded biological material provides valuable insights into the organellar organization of cells. With the advent of automated data-acquisition procedures, it has become possible to study radiation-sensitive specimens, such as frozen-hydrated organelles and whole cells. This opens up exciting perspectives for visualizing the molecular organization of the cytoplasm.
对塑料包埋生物材料薄片进行电子断层扫描，能为细胞的细胞器组织提供有价值的见解。随着自动数据采集程序的出现，研究辐射敏感标本（如冷冻水合细胞器和完整细胞）已成为可能。这为可视化细胞质的分子组织开辟了令人兴奋的前景。
