Lendvai Balazs, Zelles Tibor, Rozsa Balazs, Vizi E Sylvester
Institute of Experimental Medicine, Hungarian Academy of Sciences, Budapest, Hungary.
Brain Res Bull. 2003 Jan 15;59(4):257-60. doi: 10.1016/s0361-9230(02)00873-0.
We imaged neocortical layer 2/3 pyramidal cells in rat brain slices with two-photon laser scanning microscopy to investigate that spine motility can be influenced by the voltage-dependent Na(+) and Ca(2+) channel inhibitor, vinpocetine, which exhibited positive cognitive effects in human studies. Veratridine, which enhances sodium influx, was also tested on dendritic spine motility. Perfusion with vinpocetine, a derivative of vinca alkaloids, caused a substantial increase in the structural dynamics of dendritic spines measured by the changes in length or the number of new/retracted spines. In contrast, enhancement of sodium influx with veratridine failed to change spine motility. Our results indicate that the rapid changes in spine shape and size could occur, when calcium and sodium influx has been decreased by this vinca alkaloid. Spine motility induced by vinpocetine may be associated to microtubule alterations, an effect that was described for other vinca alkaloids. On the other hand, the potential of vinpocetine to enhance cognition in clinical studies suggests that the increased spine motility may be related to cognitive functions.
我们使用双光子激光扫描显微镜对大鼠脑片的新皮层第2/3层锥体细胞进行成像，以研究脊柱运动性是否会受到电压依赖性钠通道和钙通道抑制剂长春西汀的影响，该药物在人体研究中显示出积极的认知效果。还对增强钠内流的藜芦碱对树突棘运动性进行了测试。用长春西汀（一种长春花生物碱的衍生物）灌注，通过长度变化或新/缩回棘的数量变化来衡量，导致树突棘的结构动力学显著增加。相比之下，用藜芦碱增强钠内流未能改变棘的运动性。我们的结果表明，当这种长春花生物碱减少钙和钠内流时，棘的形状和大小可能会迅速变化。长春西汀诱导的棘运动性可能与微管改变有关，其他长春花生物碱也有这种作用。另一方面，长春西汀在临床研究中增强认知的潜力表明，增加的棘运动性可能与认知功能有关。
