Binesh Nader, Yue Kenneth, Fairbanks Lynn, Thomas M Albert
Department of Radiological Sciences, UCLA School of Medicine, University of California-Los Angeles, Los Angeles, California 90095-1721, USA.
Magn Reson Med. 2002 Dec;48(6):942-8. doi: 10.1002/mrm.10307.
The test-retest reliability of two-dimensional (2D) correlated spectroscopy (COSY) was studied on a whole-body 1.5T MRI scanner. Single-voxel localized 2D proton spectra were recorded in vitro as well as in vivo using a recently implemented localized chemical shift correlated spectroscopic (L-COSY) sequence. A total of 40 in vitro and 40 human brain (10 volunteers, four times each) 2D L-COSY spectra were recorded. The coefficients of variation (CVs) of selected brain metabolites (raw volume integrals) recorded in 10 healthy volunteers were less than 9% for creatine, choline, and N-acetyl aspartate, and less than 17% for myo-inositol, glutamine/glutamate, aspartate, and threonine/lactate. The 2D metabolite ratios and the raw volume integrals of 2D diagonal and cross peaks in healthy human brain were very well reproduced. The intraclass correlation coefficients were greater than 0.4 (P < 0.05) for the major metabolites, indicating that the 2D peak volumes were stable enough within individuals to detect reliable differences between normal subjects.
在一台全身1.5T磁共振成像（MRI）扫描仪上研究了二维（2D）相关光谱学（COSY）的重测信度。使用最近实施的局部化学位移相关光谱学（L-COSY）序列在体外以及体内记录单像素局部二维质子光谱。总共记录了40个体外和40个人脑（10名志愿者，每人4次）的二维L-COSY光谱。在10名健康志愿者中记录的选定脑代谢物（原始体积积分）的变异系数（CV），肌酸、胆碱和N-乙酰天门冬氨酸小于9%，肌醇、谷氨酰胺/谷氨酸、天冬氨酸和苏氨酸/乳酸小于17%。健康人脑的二维代谢物比率以及二维对角线和交叉峰的原始体积积分得到了很好的重现。主要代谢物的组内相关系数大于0.4（P<0.05），表明二维峰体积在个体内足够稳定，能够检测正常受试者之间可靠的差异。
