Ho Cheong-Yip, Wong Chun-Kwok, Li Edmund Kwok-Ming, Tam Lai-Shan, Lam Christopher Wai-Kei
Department of Chemical Pathology, The Chinese University of Hong Kong, Prince of Wales Hospital, Shatin, NT, Hong Kong.
J Rheumatol. 2002 Dec;29(12):2537-44.
The roles of cyclins and cyclin dependent kinase (CDK) inhibitors of lymphocytes in the pathogenesis of systemic lupus erythematosus (SLE) are unclear. We measured the expression of cyclin B1 and CDK inhibitor p21 in peripheral blood lymphocytes (PBL) from patients with SLE and controls.
PBL from 40 SLE patients with renal disease (RSLE), 40 SLE patients without renal disease (SLE), and 28 healthy control subjects were cultured with phytohemagglutinin (PHA). Bivariate distribution of cyclin B1 or p21 expression versus cellular DNA content was assessed by flow cytometry.
Expression of p21 in lymphocytes was significantly lower in patients with RSLE and with SLE than controls (RSLE vs controls and SLE vs controls, both p < 0.001). Expression of cyclin B1 was similar in all groups. The percentages of RSLE lymphocytes in G0/G1 and S phase were significantly reduced and elevated, respectively, compared with controls.
Downregulated p21 in PHA stimulated PBL from patients with SLE may be closely related to aberration of cell division in SLE lymphocytes.
淋巴细胞周期蛋白及周期蛋白依赖性激酶（CDK）抑制剂在系统性红斑狼疮（SLE）发病机制中的作用尚不清楚。我们检测了SLE患者和对照者外周血淋巴细胞（PBL）中周期蛋白B1和CDK抑制剂p21的表达。
用植物血凝素（PHA）培养40例患有肾脏疾病的SLE患者（RSLE）、40例无肾脏疾病的SLE患者（SLE）以及28名健康对照者的PBL。通过流式细胞术评估周期蛋白B1或p21表达与细胞DNA含量的双变量分布。
RSLE患者和SLE患者淋巴细胞中p21的表达显著低于对照者（RSLE与对照者相比以及SLE与对照者相比，均p < 0.001）。所有组中周期蛋白B1的表达相似。与对照者相比，RSLE淋巴细胞在G0/G1期和S期的百分比分别显著降低和升高。
SLE患者PHA刺激的PBL中p21表达下调可能与SLE淋巴细胞的细胞分裂异常密切相关。
