Barrie E, Heath D, Stella J A, Harris P
Environ Physiol Biochem. 1975;5(1):18-26.
The activities of several enzymes functioning in different areas of fuel catabolism were measured under standardized conditions, using crude homogenates of sartorius and ventricular muscle from outbred guinea-pigs and rabbits indigenous to high or low altitude. The activities of sartorius and myocardium were found to reflect the metabolic patterns known to be associated with white and red muscle. Both species had right ventricular hypertrophy at high altitude. The enzyme activities in the high altitude guinea-pig were not significantly different from those in the sea level animals. In the high altitude rabbit, compared with the low altitude rabbit, the activities of glyceraldehyde-3-phosphate deydrogenase and phosphofructokinase were greater in both the sartorius and myocardium. In addition, mitochondrial glycerol-3-phosphate dehydrogenase activity was greater in the sartorius at high altitude, while aspartate aminotransferase and beta-hydroxyacylcoenzyme A dehydrogenase activities were greater in the myocardium at high altitude. Succinate dehydrogenase activity was comparable at the two altitudes for both tissues. There was a greater proportion of skeletal muscle type lactate dehydrogenase in the high altitude rabbit myocardium but no difference was found with the guinea-pig.
在标准化条件下，使用来自高海拔或低海拔地区的杂种豚鼠和兔子的缝匠肌和心室肌的粗匀浆，测量了在燃料分解代谢不同区域发挥作用的几种酶的活性。发现缝匠肌和心肌的活性反映了已知与白肌和红肌相关的代谢模式。两种物种在高海拔地区均出现右心室肥大。高海拔豚鼠的酶活性与海平面动物的酶活性没有显著差异。与低海拔兔子相比，高海拔兔子的缝匠肌和心肌中甘油醛-3-磷酸脱氢酶和磷酸果糖激酶的活性更高。此外，高海拔地区缝匠肌中线粒体甘油-3-磷酸脱氢酶的活性更高，而高海拔地区心肌中天冬氨酸转氨酶和β-羟酰基辅酶A脱氢酶的活性更高。两种组织在两个海拔高度的琥珀酸脱氢酶活性相当。高海拔兔子心肌中骨骼肌型乳酸脱氢酶的比例更高，但豚鼠没有差异。
