Vereycken-Holler V, Aigueperse J, Gaugler M H
Institut de Radioprotection et de Sureté Nucléaire, Département de Protection de la santé de l'Homme et de Dosimétrie, Section Autonome de Radiobiologie Appliquée à la Médecine, IRSN, B.P. no. 17, F-92262 Fontenay-aux-Roses cedex, France.
Int J Radiat Biol. 2002 Oct;78(10):923-30. doi: 10.1080/09553000210152980.
To quantify in vitro the functional consequences of irradiation on the interactions between leukocytes or platelets and endothelial cells (EC) in flowing whole blood using a parallel-plate flow chamber and real-time videomicroscopy.
The parallel-plate flow chamber was calibrated to determine the dynamic parameters of the flow channel. Fluorescent-labelled whole blood was perfused at wall shear rates of 25, 75 and 500 s(-1) over a monolayer of human microvascular EC-lung (HMVEC-L) with or without irradiation at 10 Gy. The adhesion of leukocytes and platelets on EC was quantified by videomicroscopy and image analysis.
Calibration of the parallel-plate flow chamber showed that flow in the chamber was laminar and steady and had a parabolic velocity profile, thus simulating physiological flow conditions. Flow assay revealed that rolling, mean rolling velocity and firm adhesion of leukocytes was increased following irradiation of EC. Irradiation also favoured platelet adhesion to EC.
The results of an in vitro flow assay with whole blood showed that under physiological flow conditions, irradiation affected the function of EC; pro-inflammatory and thrombogenic responses were enhanced, which may contribute to in vivo radiation-induced vascular occlusion and fibrosis.
使用平行板流动腔和实时视频显微镜在体外定量研究照射对全血中白细胞或血小板与内皮细胞（EC）相互作用的功能影响。
对平行板流动腔进行校准以确定流动通道的动态参数。将荧光标记的全血以25、75和500 s(-1)的壁面剪切速率灌注在经或未经10 Gy照射的人微血管内皮细胞-肺（HMVEC-L）单层上。通过视频显微镜和图像分析对白细胞和血小板在EC上的黏附进行定量。
平行板流动腔的校准表明，腔内流动呈层流且稳定，具有抛物线速度分布，从而模拟了生理流动条件。流动分析显示，照射EC后白细胞的滚动、平均滚动速度和牢固黏附增加。照射也有利于血小板黏附于EC。
全血体外流动分析结果表明，在生理流动条件下，照射影响EC的功能；促炎和血栓形成反应增强，这可能导致体内辐射诱导的血管闭塞和纤维化。
