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KIAA基因小鼠同源物编码序列的预测：I. 通过筛选从大小分级文库中随机取样的cDNA克隆的末端序列鉴定出的100个小鼠KIAA同源cDNA的完整核苷酸序列。

Prediction of the coding sequences of mouse homologues of KIAA gene: I. The complete nucleotide sequences of 100 mouse KIAA-homologous cDNAs identified by screening of terminal sequences of cDNA clones randomly sampled from size-fractionated libraries.

作者信息

Okazaki Noriko, Kikuno Reiko, Ohara Reiko, Inamoto Susumu, Hara Yasuhiro, Nagase Takahiro, Ohara Osamu, Koga Hisashi

机构信息

Kazusa DNA Research Institute, 2-6-7 Kazusa-Kamatari, Kisarazu, Chiba 292-0818, Japan.

出版信息

DNA Res. 2002 Oct 31;9(5):179-88. doi: 10.1093/dnares/9.5.179.

DOI:10.1093/dnares/9.5.179
PMID:12465718
Abstract

We have been conducting a human cDNA project to predict protein-coding sequences in long cDNAs (> 4 kb) since 1994. The number of these newly identified human genes exceeds 2000 and these genes are known as KIAA genes. As an extension of this project, we herein report characterization of cDNAs derived from mouse KIAA-homologous genes. A primary aim of this study was to prepare a set of mouse. KIAA-homologous cDNAs that could be used to analyze the physiological roles of KIAA genes in mice. In addition, comparison of the structures of mouse and human KIAA cDNAs might enable us to evaluate the integrity of KIAA cDNAs more convincingly. In this study, we selected mouse KIAA-homologous cDNA clones to be sequenced by screening a library of terminal sequences of mouse cDNAs in size-fractionated libraries. We present the entire sequences of 100 cDNA clones thus selected and predict their protein-coding sequences. The average size of the 100 cDNA sequences reached 5.1 kb and that of mouse KIAA-homologous proteins predicted from these cDNAs was 989 amino acid residues.

摘要

自1994年以来，我们一直在开展一项人类cDNA项目，以预测长cDNA（>4 kb）中的蛋白质编码序列。这些新鉴定出的人类基因数量超过2000个，这些基因被称为KIAA基因。作为该项目的延伸，我们在此报告源自小鼠KIAA同源基因的cDNA的特征。本研究的一个主要目的是制备一组小鼠KIAA同源cDNA，可用于分析KIAA基因在小鼠中的生理作用。此外，比较小鼠和人类KIAA cDNA的结构可能使我们能够更令人信服地评估KIAA cDNA的完整性。在本研究中，我们通过在大小分级文库中筛选小鼠cDNA末端序列文库，选择了小鼠KIAA同源cDNA克隆进行测序。我们展示了如此选择的100个cDNA克隆的完整序列，并预测了它们的蛋白质编码序列。这100个cDNA序列的平均大小达到5.1 kb，从这些cDNA预测的小鼠KIAA同源蛋白的平均大小为989个氨基酸残基。

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引用本文的文献

1
HUGE: a database for human KIAA proteins, a 2004 update integrating HUGEppi and ROUGE.HUGE：一个人类KIAA蛋白数据库，2004年更新版整合了HUGEppi和ROUGE。
Nucleic Acids Res. 2004 Jan 1;32(Database issue):D502-4. doi: 10.1093/nar/gkh035.