Wang Zhi-Fei, Zhou Jin, Tang Xi-Can
State Key Laboratory of Drug Research, Shanghai Institute of Materia Medica, Shanghai Institutes for Biological Sciences, Chinese Academy of Sciences, Shanghai 200031, China.
Acta Pharmacol Sin. 2002 Dec;23(12):1193-8.
To test the ability of huperzine B (HupB) to alleviate injury from oxygen-glucose deprivation (OGD) in the rat pheochromocytoma line PC12 cells.
After OGD for 3 h and reoxygenation for 24 h, neuronal morphology was observed by phase-contrast microscopy; cell survival was quantified by the reduction of MTT; malondialdehyde (MDA) was determined by the thiobarbituric acid; superoxide dismutase (SOD) was assayed by a modification of the xanthine/xanthine oxidase; and lactate (LA) was measured according to Marbach and Weil.
OGD for 3 h and reoxygenation for 24 h triggered death in nearly 70 % of cells, along with major changes in morphology and biochemistry including elevated level of MDA, SOD activity, and LA content. Cells pretreated with HupB 1-100 micromol/L for 2 h showed significantly improved survival and reduced biochemical and morphologic signs of toxicity.
HupB protected PC12 cells against OGD-induced injury, most likely by alleviating disturbances of oxidative and energy metabolism.
检测石杉碱乙（HupB）减轻大鼠嗜铬细胞瘤细胞系PC12细胞氧糖剥夺（OGD）损伤的能力。
在OGD 3小时和复氧24小时后，通过相差显微镜观察神经元形态；通过MTT还原法对细胞存活率进行定量；用硫代巴比妥酸法测定丙二醛（MDA）；用改良的黄嘌呤/黄嘌呤氧化酶法测定超氧化物歧化酶（SOD）；并根据马尔巴赫和韦尔的方法测量乳酸（LA）。
OGD 3小时和复氧24小时导致近70%的细胞死亡，同时伴有形态和生化方面的主要变化，包括MDA水平升高、SOD活性和LA含量增加。用1 - 100微摩尔/升的HupB预处理细胞2小时，可显著提高细胞存活率，并减少毒性的生化和形态学指标。
HupB保护PC12细胞免受OGD诱导的损伤，最可能是通过减轻氧化和能量代谢紊乱来实现的。
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