信使核糖核酸诱导培养的心肌成肌细胞中快速钠离子通道的产生

Messenger RNA induction of fast sodium ion channels in cultured cardiac myoblasts.

作者信息

McLean M J, Renaud J, Sperelakis N, Niu M C

出版信息

Science. 1976 Jan 23;191(4224):297-9. doi: 10.1126/science.1246615.

Abstract

Incubation with adult heart messenger RNA caused the appearance of fast sodium ion channels in young myocardial cells whose development has been arrested in vitro. The induction was blocked by cycloheximide, indicating dependence on protein synthesis. Thus, cardiac myoblasts can be made to differentiate in vitro, and membrane properties can be altered by exogenous RNA.

摘要

与成年心脏信使核糖核酸（mRNA）共同孵育，可使体外发育停滞的幼小心肌细胞出现快速钠离子通道。这种诱导作用被放线菌酮阻断，表明其依赖蛋白质合成。因此，心肌成纤维细胞可在体外实现分化，且膜特性可被外源RNA改变。