胚胎心脏细胞聚集体中快速钠离子电导的体外分化
In vitro differentiation of a fast Na+ conductance in embryonic heart cell aggregates.
作者信息
Nathan R D, DeHaan R L
出版信息
Proc Natl Acad Sci U S A. 1978 Jun;75(6):2776-80. doi: 10.1073/pnas.75.6.2776.
Abstract
Aggregates of 3-day chick cardiac myocytes, after 2 days of gyration culture, lack a tetrodotoxin-sensitive, fast Na+ conductance. After 2--3 additional days in vitro these aggregates were shown by three different criteria to have developed such a conductance mechanism: (a) action potential upstroke velocities greater than 90 V/sec; (b) suppression of spontaneous activity by tetrodotoxin; and (c) presence of a fast component of inward current recorded during voltage clamp. These membrane properties were similar to those of aggregates derived from 7-day hearts that had developed in ovo. The differentiation of fast Na+ conductance channels was a dependent upon active protein synthesis but not upon the presence of exogenous macromolecules.
摘要
3日龄鸡心肌细胞聚集体在旋转培养2天后，缺乏对河豚毒素敏感的快速Na⁺电导。在体外再培养2 - 3天后，通过三种不同标准表明这些聚集体已形成这样一种电导机制：(a)动作电位上升速度大于90V/秒；(b)河豚毒素抑制自发活动；(c)电压钳记录期间存在内向电流的快速成分。这些膜特性与在卵内发育的7日龄心脏来源的聚集体相似。快速Na⁺电导通道的分化依赖于活性蛋白质合成，但不依赖于外源性大分子的存在。
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