感染肠出血性大肠杆菌菌株的患者产生识别位于大肠杆菌核心脂多糖R3区域表位的血清抗体。

Production of serum antibodies that recognise epitopes located on the R3 region of Escherichia coli core lipopolysaccharides by patients infected with strains of enterohaemorrhagic E. coli.

作者信息

Chart Henrik, Cheasty Thomas, Willshaw Geraldine A

机构信息

Laboratory of Enteric Pathogens, Division of Gastrointestinal Infections, Central Public Health Laboratory, 61 Colindale Avenue, London NW9 5HT.

出版信息

J Med Microbiol. 2002 Dec;51(12):1050-1054. doi: 10.1099/0022-1317-51-12-1050.

DOI:10.1099/0022-1317-51-12-1050

PMID:12466402

Abstract

Antibody-antigen cross-reactions were examined with sera from patients with Escherichia coli O157 infection and lipopolysaccharide (LPS) purified from a range of enterohaemorrhagic E. coli (EHEC) including those belonging to serogroups O26, O103, O111, O145 and O157. Six of 10 patients infected with an O157 EHEC produced serum antibodies that cross-reacted with common LPS-core epitopes, which were expressed by 23 of 33 strains of EHEC examined. These common LPS-core epitopes were also present on strains of E. coli O26 which did not produce verocytotoxin. These cross-reacting antibodies did not influence the basic immunoblotting procedures used for the routine serodiagnosis of infections with E. coli O157.

摘要

利用感染大肠杆菌O157患者的血清以及从一系列肠出血性大肠杆菌（EHEC）中纯化得到的脂多糖（LPS）（包括属于O26、O103、O111、O145和O157血清群的菌株）检测抗体-抗原交叉反应。10例感染O157 EHEC的患者中有6例产生了与常见LPS核心表位发生交叉反应的血清抗体，在所检测的33株EHEC中有23株表达这些表位。这些常见的LPS核心表位也存在于不产生志贺毒素的大肠杆菌O26菌株中。这些交叉反应抗体不影响用于大肠杆菌O157感染常规血清诊断的基本免疫印迹程序。