Clark C G, Johnson S, Johnson R P
Agriculture and Agri-Food Canada, Health of Animals Laboratory, Guelph, Ontario.
J Med Microbiol. 1995 Oct;43(4):262-9. doi: 10.1099/00222615-43-4-262.
Monoclonal antibody (MAb) 4E8C12 has been previously reported to recognise low mol. wt proteins from enterohaemorrhagic Escherichia coli (EHEC) serotypes O157:H7 and O26:H11. Crude lipopolysaccharide (LPS) preparations from proteinase K-digested bacterial suspensions reacted in Western blots with MAb 4E8C12, as did highly purified LPS from O157:H7 strains. The material recognised by this antibody was, therefore, LPS. The LPS epitope was identified by a whole-cell ELISA in several EHEC, verotoxin producing E. coli (VTEC) and verotoxin-negative strains in addition to E. coli serotypes O157:H7 and O26:H11. Acriflavine and bile salts enhanced the production or availability of the epitope at the cell surface and in culture supernates. These data indicate that the presence of the epitope did not correlate with the virulence of these organisms.
单克隆抗体（MAb）4E8C12先前已被报道可识别肠出血性大肠杆菌（EHEC）血清型O157:H7和O26:H11的低分子量蛋白质。来自蛋白酶K消化的细菌悬液的粗脂多糖（LPS）制剂在蛋白质印迹中与单克隆抗体4E8C12发生反应，O157:H7菌株的高度纯化LPS也是如此。因此，该抗体识别的物质是LPS。通过全细胞ELISA在几种EHEC、产志贺毒素大肠杆菌（VTEC）和志贺毒素阴性菌株以及大肠杆菌血清型O157:H7和O26:H11中鉴定了LPS表位。吖啶黄素和胆盐可增强表位在细胞表面和培养上清液中的产生或可用性。这些数据表明表位的存在与这些生物体的毒力无关。
