Dahourou George, Guillot Sophie, Le Gall Olivier, Crainic Radu
Epidémiologie Moléculaire des Entérovirus, Institut Pasteur, Paris, France1.
IPV, IBVM, INRA Bordeaux-Aquitaine, BP 81, 33883 Villenave d'Ornon Cedex, France2.
J Gen Virol. 2002 Dec;83(Pt 12):3103-3110. doi: 10.1099/0022-1317-83-12-3103.
Poliovirus isolates were screened for recombinants by combined analysis of two distant polymorphic segments of the poliovirus genome (one in the capsid and the other in the polymerase-coding region). Using a restriction fragment length polymorphism (RFLP) assay, a high number of recombinant genomes was found among vaccine-derived strains excreted by poliovirus vaccine vaccinees or vaccine-associated paralytic poliomyelitis cases. Some of these subjects carried a wild-type poliovirus (non-vaccine-specific) nucleotide sequence in the 3' part of the genome. Using a similar approach, a collection of wild-type poliovirus strains isolated in South India between 1985 and 1993 was screened for recombinants. Genotypes were defined by the parallel application of RFLP assays and genomic sequencing of the capsid protein VP1 and the 3D polymerase polypeptide. Analyses revealed several instances where the position of an isolate on the phylogenic tree for the capsid protein-coding segment did not agree with its position on the tree for the polymerase-coding region. In this way, several wild-type/wild-type and wild-type/vaccine recombinants could be identified, indicating that recombination is encountered commonly in the natural evolution of poliovirus strains.
通过对脊髓灰质炎病毒基因组的两个远距离多态性片段（一个在衣壳中，另一个在聚合酶编码区）进行联合分析，筛选脊髓灰质炎病毒分离株中的重组体。使用限制性片段长度多态性（RFLP）检测法，在脊髓灰质炎疫苗接种者或疫苗相关麻痹性脊髓灰质炎病例排出的疫苗衍生毒株中发现了大量重组基因组。其中一些受试者在基因组的3'部分携带野生型脊髓灰质炎病毒（非疫苗特异性）核苷酸序列。使用类似方法，对1985年至1993年间在印度南部分离的野生型脊髓灰质炎病毒株进行了重组体筛选。通过并行应用RFLP检测法以及衣壳蛋白VP1和3D聚合酶多肽的基因组测序来确定基因型。分析揭示了几个实例，即分离株在衣壳蛋白编码片段的系统发育树上的位置与其在聚合酶编码区的树上的位置不一致。通过这种方式，可以鉴定出几种野生型/野生型和野生型/疫苗重组体，这表明在脊髓灰质炎病毒株的自然进化过程中重组很常见。
