Kahng H-Y, Nam K, Kukor J J, Yoon B-J, Lee D-H, Oh D-C, Kam S-K, Oh K-H
Department of Environmental Education, Research Institute for Basic Sciences, Cheju National University, Jeju 690-756, Korea.
Appl Microbiol Biotechnol. 2002 Dec;60(4):475-80. doi: 10.1007/s00253-002-1137-y. Epub 2002 Oct 12.
Pseudomonas rhodesiae KK1 was isolated from a former manufactured-gas plant site, due to its ability to grow rapidly in a mixture of polycyclic aromatic hydrocarbons (PAHs). Radiorespirometric analysis revealed that strain KK1 was found to be able to mineralize anthracene, naphthalene and phenanthrene. Notably, phenanthrene-grown cells were able to mineralize anthracene much more rapidly than naphthalene-grown cells. Comparative analysis of amino acid sequences from 17 randomly selected dioxygenases capable of hydroxylating unactivated aromatic nuclei indicated that the enzymes for catabolism of PAHs, such as naphthalene and phenanthrene, might exist redundantly in strain KK1. Northern hybridization for cells grown on naphthalene or phenanthrene, using the putative naphthalene or phenanthrene dioxygenase gene fragment as a probe, suggested that the enzyme for naphthalene catabolism might share some homology in deduced amino acid sequences with phenanthrene dioxygenases. Also, it was found that three lipids (17:0 cyclo, 18:1 omega7c, 19:0 cyclo) increased in response to both naphthalene and phenanthrene, while the shift of other lipids varied from substrate to substrate.
罗得西亚假单胞菌KK1是从一个以前的煤气厂遗址分离出来的，因为它能够在多环芳烃（PAHs）混合物中快速生长。放射性呼吸测定分析表明，菌株KK1能够将蒽、萘和菲矿化。值得注意的是，以菲为碳源生长的细胞比以萘为碳源生长的细胞能更快地将蒽矿化。对17种随机选择的能够羟基化未活化芳香核的双加氧酶的氨基酸序列进行比较分析表明，在菌株KK1中，可能存在多种用于多环芳烃（如萘和菲）分解代谢的酶。以推测的萘或菲双加氧酶基因片段为探针，对以萘或菲为碳源生长的细胞进行Northern杂交，结果表明，萘分解代谢酶在推导的氨基酸序列上可能与菲双加氧酶有一些同源性。此外，还发现三种脂质（17:0环丙烷、18:1 ω7c、19:0环丙烷）对萘和菲都有响应而增加，而其他脂质的变化则因底物而异。
