Clarkin S D, Clesceri L S
Rensselaer Polytechnic Institute, Biology Department, Troy, NY 12180, USA.
Appl Microbiol Biotechnol. 2002 Dec;60(4):485-8. doi: 10.1007/s00253-002-1138-x. Epub 2002 Oct 12.
Commercial celluloses (BH20, Epicote, FC+) and their cellulose-containing powdered mixed resins (PMR) were evaluated using enzymatic and physical methods. Samples were hydrolyzed with purified Trichoderma viride cellulase extract and measured for released reducing sugar using the dinitrosalicylic acid method. Physical characterization was performed with gross specific surface areas (GSSA) and relative crystalline indices (RCI). In addition, FC+ was exposed to physical and chemical processing commonly encountered in spent PMR processing to determine potential effects on reducing sugar release in high intensity containers. Reducing sugar released from the celluloses by T. viride cellulase ranged from 135.37 to 244.48 mg day(-1); the celluloses were highly crystalline, ranging from 82.47 to 84.57%; and the GSSA medians for the celluloses ranged from 1,298.60 cm(2) g(-1) to 2,493.20 cm(2) g(-1). Most processing treatments on the FC+ reduced the amount of reducing sugar released and increased RCI. Cellulose hydrolysis rates did not show a strong correlation with the physical characterization. These results suggest that (1) celluloses and PMR can serve as abundant sources of bioavailable carbon in water treatment systems, and (2) the use of correlative physical characteristics to evaluate a cellulose-based commercial product may not accurately predict microbial activity; a complementary microbial test such as cellulose hydrolysis with cellulase may prove useful.
使用酶法和物理方法对商用纤维素（BH20、Epicote、FC+）及其含纤维素的粉末状混合树脂（PMR）进行了评估。样品用纯化的绿色木霉纤维素酶提取物水解，并用二硝基水杨酸法测量释放的还原糖。通过总比表面积（GSSA）和相对结晶指数（RCI）进行物理表征。此外，将FC+暴露于废旧PMR处理中常见的物理和化学处理条件下，以确定其对高强度容器中还原糖释放的潜在影响。绿色木霉纤维素酶从纤维素中释放的还原糖范围为135.37至244.48毫克/天；这些纤维素具有高度结晶性，范围为82.47%至84.57%；纤维素的GSSA中位数范围为1298.60平方厘米/克至2493.20平方厘米/克。对FC+进行的大多数处理都减少了还原糖的释放量并提高了RCI。纤维素水解速率与物理表征没有很强的相关性。这些结果表明：（1）纤维素和PMR可作为水处理系统中生物可利用碳的丰富来源；（2）使用相关物理特性来评估纤维素基商业产品可能无法准确预测微生物活性；补充性的微生物测试，如用纤维素酶进行纤维素水解测试，可能会很有用。
