Klésov A A
Biokhimiia. 1982 Apr;47(4):608-18.
A kinetic analysis of the main regularities for formation and consumption of the intermediate metabolites (i.e. cellooligosaccharides and cellobiose) in the course of enzymatic hydrolysis of cellulose has been performed. The boundary relationships between the velocities of the individual steps of hydrolysis controlling the transition of the multienzyme cellulase system to be steady-state with respect to the intermediate metabolites were revealed. Using the kinetic approaches developed here, the values of the steady-state concentrations of insoluble intermediate oligosaccharides were determined experimentally for a number of cellulases from the fungi Trichoderma and Geotrichum. It was shown experimentally that when studying the kinetics of enzymatic hydrolysis of cellulose it is necessary to take into account the structural irregularities of the insoluble substrate even when a practically negligible fraction of it (with respect to its weight) is converted to soluble products. In other cases the kinetic parameters obtained could not be corresponding to the actual reactivity of the cellulose/cellulase system, reflecting rather a difference in reactivity between the labile (amorphous) and stable (crystalline) fractions of a cellulose substrate.
对纤维素酶解过程中中间代谢产物（即纤维寡糖和纤维二糖）形成和消耗的主要规律进行了动力学分析。揭示了控制多酶纤维素酶系统相对于中间代谢产物转变为稳态的水解各个步骤速度之间的边界关系。利用此处开发的动力学方法，对来自木霉属和地霉属真菌的多种纤维素酶，通过实验测定了不溶性中间寡糖的稳态浓度值。实验表明，在研究纤维素酶解动力学时，即使不溶性底物转化为可溶性产物的部分实际上可以忽略不计（相对于其重量），也有必要考虑不溶性底物的结构不规则性。在其他情况下，获得的动力学参数可能与纤维素/纤维素酶系统的实际反应性不对应，而更多地反映了纤维素底物不稳定（无定形）和稳定（结晶）部分之间反应性的差异。
