Sheu Dey-Chyi, Duan Kow-Jen, Cheng Chih-Yu, Bi Jia-Lin, Chen Jer-Yi
Department of Bioengineering, Tatung University, Taipei 10451, Taiwan.
Biotechnol Prog. 2002 Nov-Dec;18(6):1282-6. doi: 10.1021/bp020081y.
A complex biocatalyst system with a bioreactor equipped with a microfiltration (MF) module was employed to produce high-content fructooligosaccharides (FOS) in a continuous process initiated by a batch process. The system used mycelia of Aspergillus japonicus CCRC 93007 or Aureobasidium pullulans ATCC 9348 with beta-fructofuranosidase activity and Gluconobacter oxydans ATCC 23771 with glucose dehydrogenase activity. Calcium carbonate slurry was used to control pH to 5.5, and gluconic acid in the reaction mixture was precipitated as calcium gluconate. Sucrose solution with an optimum concentration of 30% (w/v) was employed as feed for the complex cell system, and high-content FOS was discharged continuously from a MF module. The complex cell system was run at 30 degrees C with an aeration rate of 5 vvm and produced more than 80% FOS with the remainder being 5-7% glucose and 8-10% sucrose on a dry weight basis, plus a small amount of calcium gluconate. The system worked for a 7-day continuous production process with a dilution rate of 0.04 h(-1), and the volumetric productivity for total FOS was more than 160 g L(-1) h(-1).
采用一个配备微滤（MF）模块的生物反应器组成的复杂生物催化剂系统，在由分批过程启动的连续过程中生产高含量的低聚果糖（FOS）。该系统使用具有β-呋喃果糖苷酶活性的日本曲霉CCRC 93007或出芽短梗霉ATCC 9348的菌丝体，以及具有葡萄糖脱氢酶活性的氧化葡萄糖杆菌ATCC 23771。使用碳酸钙浆液将pH控制在5.5，反应混合物中的葡萄糖酸沉淀为葡萄糖酸钙。以最佳浓度30%（w/v）的蔗糖溶液作为复合细胞系统的进料，高含量的FOS从MF模块中连续排出。复合细胞系统在30℃下运行，通气速率为5 vvm，以干重计，产生超过80%的FOS，其余为5 - 7%的葡萄糖和8 - 10%的蔗糖，外加少量葡萄糖酸钙。该系统以0.04 h⁻¹的稀释率进行了为期7天的连续生产过程，总FOS的体积生产率超过160 g L⁻¹ h⁻¹。
