Yoshikawa Jun, Amachi Seigo, Shinoyama Hirofumi, Fujii Takaaki
Department of Advanced Bioresources Science, Graduate School of Science and Technology, Chiba University, Matsudo-City, Chiba, Japan.
Biotechnol Lett. 2008 Mar;30(3):535-9. doi: 10.1007/s10529-007-9568-2. Epub 2007 Oct 30.
Fructooligosaccharides (FOS) were produced from sucrose by using crude enzyme preparations of beta-fructofuranosidases (FFases) obtained from sucrose-cultured cells of Aureobasidium pullulans DSM 2404. When the preparation mainly consisted of FFase I, that has high transfructosylating activity, the FOS yield was 62%. When the reaction was carried out with additional commercial glucose isomerase (GI) at an activity ratio of FFase and GI of 1:2, the maximum FOS yield reached 69%. This value was higher than those obtained previously using other Aureobasidium spp. (53-59%).