Mahmud Yahia, Okada Kentaro, Takatani Tomohiro, Kawatsu Kentaro, Hamano Yonekazu, Arakawa Osamu, Noguchi Tamao
School of Science and Technology, Nagasaki University, Bunkyo-machi 1-14, 852-8521, Nagasaki, Japan.
Toxicon. 2003 Jan;41(1):13-8. doi: 10.1016/s0041-0101(02)00169-1.
Micro distribution pattern of tetrodotoxin (TTX) in several tissues of marine puffers Takifugu vermicularis and Chelonodon patoca was investigated by means of monoclonal antibody-based immunoenzymatic technique under light microscope. In the investigation TTX was visualized at glands in the skin of T. vermicularis, while in C. patoca TTX was detected in succiform cells of the skin section. Similarly, in the ovary section of T. vermicularis TTX was recognized at late peri nucleolus stage, yolk granule stage-I, and yolk granule stage-II of oocytes. The oocytes of late peri nucleolus stage and yolk granule stage-I showed TTX antigen at their nucleus and yolk vesicles, while in yolk granule stage-II TTX was visualized at yolk granules and yolk vesicles. In the ovary of C. patoca TTX was detected in the connective tissues and in the nucleus of some perinucleolar oocytes. In the liver and muscle of C. patoca TTX was found to be distributed in parenchymal hepatocytes and muscle fiber, respectively. This study, however, reveals that intra-tissue distribution of TTX varies in respect of species.
采用基于单克隆抗体的免疫酶技术，在光学显微镜下研究了虫纹东方鲀和卵形鲀几种组织中河豚毒素（TTX）的微观分布模式。在研究中，在虫纹东方鲀皮肤的腺体中观察到了TTX，而在卵形鲀的皮肤切片的杯状细胞中检测到了TTX。同样，在虫纹东方鲀的卵巢切片中，在卵母细胞的核仁后期、卵黄颗粒期I和卵黄颗粒期II检测到了TTX。核仁后期和卵黄颗粒期I的卵母细胞在其细胞核和卵黄小泡处显示出TTX抗原，而在卵黄颗粒期II，在卵黄颗粒和卵黄小泡处观察到了TTX。在卵形鲀的卵巢中，在结缔组织和一些核仁周围卵母细胞的细胞核中检测到了TTX。在卵形鲀的肝脏和肌肉中，发现TTX分别分布在实质肝细胞和肌纤维中。然而，这项研究表明，TTX在组织内的分布因物种而异。
