Utsunomiya Hirotoshi, Takekoshi Susumu, Gato Nobuki, Utatsu Hisao, Motley Evangeline D, Eguchi Kunie, Fitzgerald Trinita G, Mifune Mizuo, Frank Gerald D, Eguchi Satoru
Department of Pathology, Wakayama Medical University, Japan.
Life Sci. 2002 Dec 27;72(6):659-67. doi: 10.1016/s0024-3205(02)02300-7.
Bainiku-ekisu, the fruit-juice concentrate of the Oriental plum (Prunus mume) has recently been shown to improve human blood fluidity. We have shown that angiotensin II (AngII) stimulates growth of vascular smooth muscle cells (VSMCs) through epidermal growth factor (EGF) receptor transactivation that involves reactive oxygen species (ROS) production. To better understanding the possible cardiovascular protective effect of Bainiku-ekisu, we have studied whether Bainiku-ekisu inhibits AngII-induced growth promoting signals in VSMCs. Bainiku-ekisu markedly inhibited AngII-induced EGF receptor transactivation. H(2)O(2)-induced EGF receptor transactivation was also inhibited by Bainiku-ekisu. Thus, Bainiku-ekisu markedly inhibited AngII-induced extracellular signal-regulated kinase (ERK) activation. However, EGF-induced ERK activation was not affected by Bainiku-ekisu. AngII stimulated leucine uptake in VSMCs that was significantly inhibited by Bainiku-ekisu. Also, Bainiku-ekisu possesses a potent antioxidant activity. Since the activation of EGF receptor, ERK and the production of ROS play central roles in mediating AngII-induced vascular remodeling, these data suggest that Bainiku-ekisu could exert a powerful cardiovascular protective effect with regard to cardiovascular diseases.
梅肉提取物，即青梅（Prunus mume）的浓缩果汁，最近已被证明可改善人体血液流动性。我们已经表明，血管紧张素II（AngII）通过涉及活性氧（ROS）产生的表皮生长因子（EGF）受体反式激活来刺激血管平滑肌细胞（VSMC）的生长。为了更好地理解梅肉提取物可能的心血管保护作用，我们研究了梅肉提取物是否抑制VSMC中AngII诱导的生长促进信号。梅肉提取物显著抑制AngII诱导的EGF受体反式激活。H₂O₂诱导的EGF受体反式激活也被梅肉提取物抑制。因此，梅肉提取物显著抑制AngII诱导的细胞外信号调节激酶（ERK）激活。然而，EGF诱导的ERK激活不受梅肉提取物影响。AngII刺激VSMC中的亮氨酸摄取，而这被梅肉提取物显著抑制。此外，梅肉提取物具有强大的抗氧化活性。由于EGF受体、ERK的激活以及ROS的产生在介导AngII诱导的血管重塑中起核心作用，这些数据表明梅肉提取物在心血管疾病方面可能发挥强大的心血管保护作用。
