[海南省恶性疟原虫分离株裂殖子表面蛋白1的基因分型]

[Gene typing of merozoite surface protein 1 of Plasmodium falciparum isolates from Hainan Province].

作者信息

Jiang G F, Hong J D, Chen P Q, Wang S Q, Meng F

机构信息

Department of Medical Parasitology, Guangdong College of Pharmacy, Guangzhou 510224.

出版信息

Zhongguo Ji Sheng Chong Xue Yu Ji Sheng Chong Bing Za Zhi. 2001;19(1):4-6.

PMID:12572013

Abstract

OBJECTIVE

To identify the genotype of merozoite surface protein 1 (MSP1) of Plasmodium falciparum isolates from Hainan Province.

METHODS

Nested PCR was applied to amplify the MSP1 of Blocks 2 and 3 Plasmodium falciparum isolates from Hainan Province. Two allelic family representative gene fragments were sequenced.

RESULTS

From 36 out of 39 blood samples from Plasmodium falciparum patients, 44 gene fragments of blocks 2 and 3 of the MSP1 were amplified, of which the MAD20-type allele was dominant(75%). followed by K1-type allele. No RO33-type allele was found. The mixed infection rate of the two different allelic type was 19.4%. Sequence analysis showed that the sequences of MAD20- and K1-type isolates from Hainan Province were highly homologous to that of the MAD20 and K1 allelic prototypes.

CONCLUSION

Two principal allelic types of MSP1 gene, MAD20- and K1-type, exist in malaria endemic areas in Hainan Province, the MAD20-type being the dominant.

摘要

目的

鉴定海南省恶性疟原虫分离株裂殖子表面蛋白1（MSP1）的基因型。

方法

采用巢式聚合酶链反应（PCR）扩增海南省恶性疟原虫分离株MSP1第2和第3区。对两个等位基因家族代表性基因片段进行测序。

结果

在39份恶性疟原虫患者血样中的36份中，扩增出44条MSP1第2和第3区的基因片段，其中MAD20型等位基因占主导地位（75%），其次是K1型等位基因。未发现RO33型等位基因。两种不同等位基因类型的混合感染率为19.4%。序列分析表明，海南省MAD20型和K1型分离株的序列与MAD20和K1等位基因原型高度同源。