转化生长因子-β1和纤连蛋白对人牙周膜细胞培养中SPARC表达的影响。
Effects of transforming growth factor-beta 1 and fibronectin on SPARC expression in cultures of human periodontal ligament cells.
作者信息
Fujita Tsuyoshi, Shiba Hideki, Sakata Masatoshi, Uchida Yuushi, Ogawa Tetsuji, Kurihara Hidemi
机构信息
Department of Periodontal Medicine, Division of Frontier Medical Science, Hiroshima University Graduate School of Biomedical Sciences, 1-2-3, Kasumi, Minami-ku, 734-8553, Hiroshima, Japan.
出版信息
Cell Biol Int. 2002;26(12):1065-72. doi: 10.1006/cbir.2002.0966.
Transforming growth factor-beta(1) (TGF-beta(1)) increases synthesis of secreted protein, acidic and rich in cysteine (SPARC), as well as fibronectin (FN) and type I collagen. However, little is known about the regulatory mechanism of SPARC expression. We examined the effect of FN on SPARC expression by TGF-beta(1) in cultures of human periodontal ligament cells (HPL cells). TGF-beta(1) increased the SPARC and SPARC mRNA levels in HPL cells. Extracellular matrix (ECM) produced by HPL cells in the presence of TGF-beta(1) also increased the SPARC levels. Contents of FN and type I collagen in the ECM were increased by TGF-beta(1). HPL cells cultured on FN-coated plates secreted more SPARC than those on non-coated plates. However, type I collagen had little effect on SPARC levels. The addition of anti-alpha5 antibody to the cultures abolished the increase in SPARC mRNA expression by TGF-beta(1). This study demonstrated that FN may be partly involved in the increase in SPARC expression by TGF-beta(1) in HPL cells.
转化生长因子-β1（TGF-β1）可增加富含半胱氨酸的酸性分泌蛋白（SPARC）、纤连蛋白（FN）和I型胶原的合成。然而，关于SPARC表达的调控机制知之甚少。我们在人牙周膜细胞（HPL细胞）培养物中研究了FN对TGF-β1诱导的SPARC表达的影响。TGF-β1可增加HPL细胞中SPARC和SPARC mRNA水平。在TGF-β1存在下HPL细胞产生的细胞外基质（ECM）也可增加SPARC水平。TGF-β1可增加ECM中FN和I型胶原的含量。在FN包被的平板上培养的HPL细胞比在未包被平板上培养的细胞分泌更多的SPARC。然而，I型胶原对SPARC水平影响很小。向培养物中添加抗α5抗体可消除TGF-β1诱导的SPARC mRNA表达增加。本研究表明，FN可能部分参与了TGF-β1诱导的HPL细胞中SPARC表达的增加。
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