Asaumi Jun-Ichi, Higuchi Yuzuru, Murakami Jun, Matsuzaki Hidenobu, Wakasa Toru, Inoue Tetsuyoshi, Shigehara Hiroshi, Konouchi Hironobu, Hisatomi Miki, Kawasaki Shoji, Kuroda Masahiro, Hiraki Yoshio, Kishi Kanji
Department of Oral and Maxillofacial Radiology, Field of Tumor Biology, Graduate School of Medicine and Dentistry, Okayama University, Japan.
Oncol Rep. 2003 Jan-Feb;10(1):71-4.
We report on thermoradiotherapy combined with p53 gene therapy in the human salivary gland adenocarcinoma cell line HSG. HSG cells were successfully infected at a rate of 62.3% with MOIs of 20 of either AxCAip53 or AxCAiLacZ. The AxCAiLacZ did not inhibit the survival of the HSG cells. The survival fractions of AxCAip53-infected HSG cells were lower than those of the AxCAiLacZ-infected HSG cells, but there was no significant difference (p=0.30). AxCAip53 decreased the survival rates of thermotherapy (43 degrees C; p=0.084 and 44 degrees C; p=0.18), radiotherapy (6 Gy; p=0.20) and thermoradiotherapy (6 Gy plus 43 degrees C; p=0.24 and 6 Gy plus 44 degrees C; p=0.96), but there were no significant differences. In comparing the survival rates of thermoradiotherapy with those of thermotherapy and radiotherapy, thermoradiotherapy, regardless of the combination with p53 gene therapy, demonstrated actual survival rates lower than theoretical survival rates based on survival rates of thermotherapy multiplied by survival rates of radiotherapy. This result indicates that thermoradiotherapy is effective in the treatment of HSG cells. Thermoradiotherapy combined with p53 gene therapy was the most effective therapy among the combinations of therapies demonstrating that thermoradiotherapy combined with p53 gene therapy may be a useful tool in the treatment of HSG cells.
我们报告了热放疗联合p53基因治疗人唾液腺腺癌细胞系HSG的情况。AxCAip53或AxCAiLacZ的感染复数为20时，HSG细胞以62.3%的感染率被成功感染。AxCAiLacZ不抑制HSG细胞的存活。AxCAip53感染的HSG细胞的存活分数低于AxCAiLacZ感染的HSG细胞，但无显著差异（p = 0.30）。AxCAip53降低了热疗（43℃；p = 0.084和44℃；p = 0.18）、放疗（6 Gy；p = 0.20）和热放疗（6 Gy加43℃；p = 0.24和6 Gy加44℃；p = 0.96）的存活率，但无显著差异。在比较热放疗与热疗和放疗的存活率时，无论是否联合p53基因治疗，热放疗的实际存活率均低于基于热疗存活率乘以放疗存活率的理论存活率。这一结果表明热放疗对HSG细胞的治疗有效。热放疗联合p53基因治疗是所测试的联合治疗中最有效的治疗方法，表明热放疗联合p53基因治疗可能是治疗HSG细胞的一种有用手段。
